Abstract
A thermotolerant methylotrophicBacillus sp. (KISRI TM1A, NCIMB 40040), isolated from the Kuwaiti environment and belonging to the group II spore-forming, bacilli, could not be correlated with any knownBacillus sp. It may, therefore, be a new species. It grew at temperatures from 37° to 58°C from pH 6.5 to 9.0 and on methanol up to 40 g l−1. It grew well in a chemostat. Its biomass yield coefficient was improved by about 30% by optimization of medium and growth conditions, reaching a maximum of 0.44g g−1 at 45°C pH 6.8 to 7.0, dilution rate 0.25 h−1 with methanol at 10 g l−1. Average crude protein and amino acid content were 84% and 60%, respectively, and maximum productivity attained under laboratory conditions was 5.06 g l−1h−1. It was concluded that this strain has good potential for use in single-cell protein production.
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Banat, I.M., Murad, M. & Hamdan, I.Y. A novel thermotolerant methylotrophicBacillus sp. and its potential for use in single-cell protein production. World J Microbiol Biotechnol 8, 290–295 (1992). https://doi.org/10.1007/BF01201882
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DOI: https://doi.org/10.1007/BF01201882