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Micropropagation ofPanax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets

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Abstract

Somatic embryogenesis was induced in callus tissues derived from young flower buds ofPanax notoginseng via callus within 18 weeks of culture. The mature somatic embryos were germinated on half-strength Murashige and Skoog's (MS) medium supplemented with gibberellic acid A3(GA) and 6-benzyladenine (BA). The most suitable medium for optimal root formation proved to be MS medium supplemented with 1-naphthaleneacetic acid (NAA). Total DNA was extracted from the leaves of the regenerated plantlets ofP. notoginseng. Analysis of random-amplified polymorphic DNA (RAPD) using 21 arbitrary oligonucleotide 10-mers, showed the genetic homogeneity ofP. notoginseng. The amplification products were monomorphic for all of the plantlets ofP. notoginseng regenerated by embryogenesis, suggesting that somatic embryogenesis can be used for clonal micropropagation of this plant.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Kyushu University, 812, Higashiku, Fukuoka, Japan

    Y. Shoyama, X. X. Zhu & R. Nakai

  2. Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, 812, Higashi-ku, Fukuoka, Japan

    S. Shiraishi

  3. Institute of Pharmaceutical Sciences, Hiroshima University, School of Medicine, Kasumi 1-2-3, 734, Minami-ku, Hiroshima, Japan

    H. Kohda

Authors
  1. Y. Shoyama
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  2. X. X. Zhu
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  3. R. Nakai
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  4. S. Shiraishi
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  5. H. Kohda
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Communicated by J. M. Widholm

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Shoyama, Y., Zhu, X.X., Nakai, R. et al. Micropropagation ofPanax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets. Plant Cell Reports 16, 450–453 (1997). https://doi.org/10.1007/BF01092764

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  • DOI: https://doi.org/10.1007/BF01092764

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