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The possible role of phospholipase A2 in hepatic microsomal lipid peroxidation induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in rats

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Abstract

The induction of lipid peroxidation in hepatic microsomes of rodents treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is well documented. The potential mechanisms involved in TCDD-induced microsomal lipid peroxidation were investigated, using selected inhibitors and free radical scavengersin vitro. Rats were treated with 40 μg TCDD/kg orally as a single dose. Inhibitors of phospholipase A2, including a variety of phenothiazines, dibucaine, imipramine, and verapamil, inhibitedin vitro microsomal lipid peroxidation in response to TCDD administration. In addition, the lipoxygenase inhibitor quercetin, and the hydrogen peroxide scavenger aminopyrine inhibited lipid peroxidation with microsomes from TCDD-treated rats. The singlet oxygen scavenger β-carotene, the cytochrome P-450 substrate benzphetamine, and the cyclooxygenase inhibitor indomethacin produced moderate enhancement of hepatic microsomal lipid peroxidation. The results suggest that activation of phospholipase A2 may play a critical role in the metabolic events associated with hepatotoxicity and ultimate cell death produced by TCDD. The results also support the involvement of hydrogen peroxide in TCDD-induced microsomal lipid peroxidation.

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  1. Z. A. F. Al-Bayati

    Present address: Scientific Research Council of Iraq, Baghdad, Iraq

Authors and Affiliations

  1. School of Pharmacy and Allied Health Professions, Creighton University, 68178, Omaha, Nebraska, USA

    Z. A. F. Al-Bayati & S. J. Stohs

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  1. Z. A. F. Al-Bayati
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  2. S. J. Stohs
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Al-Bayati, Z.A.F., Stohs, S.J. The possible role of phospholipase A2 in hepatic microsomal lipid peroxidation induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in rats. Arch. Environ. Contam. Toxicol. 20, 361–365 (1991). https://doi.org/10.1007/BF01064403

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  • DOI: https://doi.org/10.1007/BF01064403

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