Abstract
Interspecific and inter-tissue differences were found in thein vitro sensitivity of oligomycin sensitive Mg2+ATPase (O.S.-ATPase) from avian mitochondrial homogenates to DDE. The enzyme from the “target” tissue, the shell gland of kestrels, which lay thin-shelled eggs on exposure to DDE, showed significantly greater sensitivity to DDE than O.S.-ATPase in muscle and brain preparations. The O.S.-ATPase from chicken shell gland was about nine times less sensitive than that from the kestrel. DDT, in contrast to DDE, was highly effective on O.S.-ATPase in all tissues tested from the three species of birds. In contrast to the high sensitivity of O.S.-ATPase, O.Ins.-ATPase and Na+-K+ATPase were not significantly affected by DDE or DDT. In addition, Ca2+-ATPases from mitochondrial and microsomal preparations from shell gland of kestrels were only minimally affected by DDT and DDE at concentrations over 20 times greater than those which inhibited O.S.-ATPase. The selectivity of DDE action in the eggshell thinning of kestrel is correlated with its action on shell gland O.S.-ATPases. However, because DDT effectively inhibits O.S.-ATPase from all tissues studied a combined effect is possible. Both would cause a reduction in ATP synthesis affecting energy-linked calcium transport. The need forin vivo studies including mitochondrial calcium transport is emphasized.
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Paper No. 11877, Scientific Journal Series, Minnesota Agricultural Experiment Station, University of Minnesota, St. Paul, MN 55108
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Khan, H.M., Cutkomp, L.K. In vitro studies of DDT, DDE, and ATPase as related to avian eggshell thinning. Arch. Environ. Contam. Toxicol. 11, 627–633 (1982). https://doi.org/10.1007/BF01056372
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DOI: https://doi.org/10.1007/BF01056372