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Expression of the humanAchaete-Scute 1 gene in olfactory neuroblastoma (esthesioneuroblastoma)

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Abstract

Olfactory neuroblastoma (ONB) is a rare neuronal malignancy of the olfactory mucosal. Markers used in the diagnosis of ONB do not distinguish ONB from other neuronal tumors or tumors with neuroendocrine features thus making the diagnosis of ONB difficult. Using a modified RT-PCR technique, we show that the human homologue of theDrosophila achaete-scute geneHASH1 is expressed in 6 primary and one metastatic ONB specimens, whereas Olfactory Marker Protein (OMP) is not. Previous studies have shown thatHASH1 is expressed in immature olfactory neurons and is required for their development.OMP, whose function is unknown, is expressed exclusively in mature olfactory neurons. Together, these data suggest that ONB is derived from immature olfactory neurons of neuroectodermal origin. Analysis of RNA expression in primary tumor specimens and in an established cell line make this an ideal system to study olfactory growth and differentiation. Furthermore, these studies represent the first molecular genetic analysis of this rare and unusual neuronal tumor.

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References

  1. O'Connor TA, McLean P, Juillard GJ, Parker RG: Olfactory neuroblastoma. Cancer 63: 2426–2428, 1989

    PubMed  Google Scholar 

  2. Taxy JB, Bharani NK, Mills SE, Frierson HF: The spectrum of olfactory tumors. A light microscopic and immunohistochemical analysis. Am J Surg Pathol 10: 687–695, 1986

    PubMed  Google Scholar 

  3. Schmidt JL, Zarbo RJ, Clark JL: Olfactory neuroblastoma: Clinicopathologic and immuno-histochemical characterization of four representative cases. Laryngoscope 100: 1052–1058, 1990

    PubMed  Google Scholar 

  4. Choi HH, Anderson PJ: Immunohistochemical diagnosis of olfactory neuroblastoma. J Neuropathol Exp Neurol 44: 18–31, 1985

    PubMed  Google Scholar 

  5. Mills SE, Frierson HF: Olfactory neuroblastoma. A clinicopathologic study of 21 cases. Am J Surg Pathol 9: 317–327, 1985

    PubMed  Google Scholar 

  6. Kadish S, Goodman M, Wang CC: Olfactory neuroblastoma. A clinical analysis of 17 cases. Cancer 37: 1571–1576, 1976

    PubMed  Google Scholar 

  7. Cantrell RW, Ghorayer BY, Fitz-Hugh GS: Esthesioneuroblastoma: diagnosis and treatment. Ann Otol 86: 760–765, 1977

    Google Scholar 

  8. Choi HH, Anderson PJ: Olfactory neuroblastoma: an immuno-electron microscopic study of S-100 protein-positive cells. J Neuropathol Exp Neurol 45: 576–587, 1986

    PubMed  Google Scholar 

  9. Llombart-Bosch A, Carda C, Peydro-Olaya S, Noguera R, Boix J, Pellin A: Pigmented esthesioneuroblastoma showing dual differentiation following transplant in nude mice. Virchow Arch A Pathol Anat 414: 199–208, 1989

    Google Scholar 

  10. Silva EG, Butler J, Mackay B, Goepfert H: Neuroblastomas and neuroendocrine carcinomas of the nasal cavity. Cancer 50: 2388–2405, 1982

    PubMed  Google Scholar 

  11. Cavazzana AO, Navarro S, Noguerra R, Reynolds PC, Triche TJ: Olfactory neuroblastoma is not neuroblastoma but is related to primitive neuroectodermal tumor (PNET). In: Evans AE, D'Angio GJ, Knudson AG, Seeger RC (eds) Advances in Neuroblastoma Research, 2. Prog Clin Biol Res, Alan R Liss, Inc., New York 463–474, 1988

    Google Scholar 

  12. Castaneda VL, Cheah MS, Saldivar VA: Cytogenetic and molecular evaluation of clinically aggressive esthesioneuroblastoma. Am J Ped Hem Onc 13: 62–70, 1991

    Google Scholar 

  13. Whang-Peng J, Freter CE, Knutsen T, Nanfro JJ, Gazdar A: Translocation t(11∶22) in esthesioneuroblastoma. Cancer Gen Cytogen 29: 155–157, 1987

    Google Scholar 

  14. Margolis FL: A marker for the olfactory chemoreceptor neuron. In: Bradshaw RA, Schneider DM (eds) Proteins of the Nervous System, Raven Press, New York 59–84, 1981

    Google Scholar 

  15. Buiakova OI, Krishna R, Getchell TV, Margolis FL: Human and rodentOMP genes: Conservation of structural and regulatory motifs and cellular localization. Genomics 20: 452–462, 1994

    PubMed  Google Scholar 

  16. Verhaagen J, Oestreicher AB, Grillo M, Khew-Goodall Y-S, Gispen WH, Margolis FL: Neuroplasticity in the olfactory system: differential effects of central and peripheral lesions of the primary olfactory pathway on the expression of B-50/GAP 43 and the olfactory marker protein. J Neurosci Res 26: 31–44, 1990

    PubMed  Google Scholar 

  17. Verhaagen J, Oestreicher AB, Gispen WH, Margolis FL: The expression of the growth associated protein B50/GAP43 in the olfactory system of neonatal and adult rats. J Neurosci 9: 683–691, 1989

    PubMed  Google Scholar 

  18. Guillemot F, Lo LC, Johnson JE, Auerbach A, Anderson DJ, Joyner AL: Mammalianachaete-scute Homolog 1 is required for the early development of olfactory and autonomic neurons. Cell 75: 463–476, 1993

    PubMed  Google Scholar 

  19. DeHarmer M, Guevara J, Hannon K: Fibroblast growth factors promote amplification divisions of olfactory receptor neuron precursors. Submitted, 1994

  20. Calof AL, Adusumalli MD, DeHarmer M: Generation, differentiation and maturation of olfactory receptor neuronsin vitro. J Cell Biochem S18B: 117, 1994

    Google Scholar 

  21. Ball DW, Azzoli CG, Baylin SB, Chi D, Dou-Keller H, Cumaraswamy A, Borges M, Nelkin B: Identification of a humanachaete-scute homolog highly expressed in neuroendocrine tumors. Proc Natl Acad Sci USA 90: 5648–5652, 1993

    PubMed  Google Scholar 

  22. Patel PI, Framson PE, Casskey T, Chinault C: Fine structure of the human hypoxanthine phosphoribosyltransferase gene. Mol Cell Biol 6: 393–403, 1986

    PubMed  Google Scholar 

  23. Erlich HA (ed): PCR Technology: Principles and Applications for DNA Amplications. Stockton Press, New York 1989

    Google Scholar 

  24. Grillo M, Margolis F: Use of reverse transcriptase polymerase chain reaction to monitor expression of intronless genes. Biotech 9: 262–268, 1990

    Google Scholar 

  25. Weizsacker FV, Labeit S, Koch HK, Oehlert W, Gerok W, Blum HE: A simple and rapid method for the detection of RNA in formalin-fixed, paraffin-embedded tissues by PCR amplification. Biochem Biophys Res Commun 174: 176–180, 1991

    PubMed  Google Scholar 

  26. Rothstein JL, Johnson D, Deloia JA, Skowronski J, Solter D, Knowles BB: Gene expression during preimplantation development. Genes Dev 6: 1190–1201, 1992

    PubMed  Google Scholar 

  27. Trojanowski JQ, Lee V, Pillsbury N, Lee S: Neuronal origin of human esthesioneuroblastoma demonstrated with antineurofilament monoclonal antibodies. N Eng J Med 307: 159–161, 1988

    Google Scholar 

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Authors and Affiliations

  1. Departments of Otolaryngology-Head and Neck Surgery and Microbiology/Immunology, Jefferson Medical College, Jefferson Cancer Institute, 233 S. 10th St., 19107, Philadelphia, PA, USA

    Mark E. Carney, Robert C. O'Reilly, Bijan Sholevar, Olesia I. Buiakova, Louis D. Lowry, William M. Keane, Frank L. Margolis & Jay L. Rothstein

  2. Roche Research Center, The Roche Institute of Molecular Biology, 07110, Nutley, NJ, USA

    Bijan Sholevar & William M. Keane

Authors
  1. Mark E. Carney
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  2. Robert C. O'Reilly
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  3. Bijan Sholevar
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  4. Olesia I. Buiakova
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  5. Louis D. Lowry
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  6. William M. Keane
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  7. Frank L. Margolis
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  8. Jay L. Rothstein
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The first two authors of this manuscript contributed equally to the study

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Carney, M.E., O'Reilly, R.C., Sholevar, B. et al. Expression of the humanAchaete-Scute 1 gene in olfactory neuroblastoma (esthesioneuroblastoma). J Neuro-Oncol 26, 35–43 (1995). https://doi.org/10.1007/BF01054767

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