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Isolation and characterization of a sialo-glycopeptide from buffalo colostrum

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Abstract

A sialoglycopeptide was isolated from buffalo colostrum in pure form by chromatography on Sephadex G-25 and QAE-Sephadex A-25. This was found to be homogeneous by cellulose acetate membrane electrophoresis and reverse phase HPLC. It consisted of fucose, galactose, mannose,N-acetyl glucosamine andN-acetyl neuraminic acid in the ratio 1∶2∶3∶4∶1, and aspartic acid, serine, threonine, proline and glutamic acid were the major amino acids. Glycine was identified as the N-terminal amino acid residue. The structure elucidation of the carbohydrate moiety was carried out by methylation analysis, mass spectrometry,1H-NMR spectroscopy and the probable structure was revealed to be that of a complex biantennary type.

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Authors and Affiliations

  1. Department of Biochemistry and Nutrition, Central Food Technological Research Institute, 570 013, Mysore, India

    Huligerepura S. Aparna & Paramahans V. Salimath

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  1. Huligerepura S. Aparna
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  2. Paramahans V. Salimath
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Aparna, H.S., Salimath, P.V. Isolation and characterization of a sialo-glycopeptide from buffalo colostrum. Glycoconjugate J 13, 63–67 (1996). https://doi.org/10.1007/BF01049681

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  • DOI: https://doi.org/10.1007/BF01049681

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