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Decrease of Normotest/Thrombotest discrepancy in non-contacted coumarin plasma after glass or ellagic acid activation

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Summary

Activation of coumarin plasmas in glass tubes for 60 min resulted in a clear shortening of Thrombotest clotting times. Normotest clotting times were shortened too but to a much lesser extent. As a consequence the Normotest (NT)/Thrombotest (TT) discrepancy which was quite large at 0 time, became progressively smaller. This phenomenon was observed both in undiluted and 6∶10 diluted plasma.

After ellagic acid activation a similar phenomenon was noted even though a less pronounced shortening of Thrombotest was noted. These data suggest that Thrombotest is very sensitive to contact phase and to factor VII activation and not to any coumarin-induced inhibitors.

Zusammenfassung

Die Aktivierung von Coumarin-Plasma in Glasröhrchen während 60 min ergab eine klare Verkürzung der Thrombotest-Gerinnungszeit. Normotest-Gerinnungszeiten wurden ebenfalls verkürzt, jedoch in einem viel geringeren Ausmaß. Infolgedessen wurde die Diskrepanz zwischen Normotest (NT) und Thrombotest (TT), die nor-malerweise ziemlich groß ist, zunehmend kleiner. Diese Erscheinung wurde sowohl in unverdünntem als auch in verdünntem (6∶10) Plasma beobachtet.

Nach einer Aktivierung durch Ellagen-Säure wurde eine ähnliche Erscheinung bemerkt, wenn auch die Verkürzung beim Thrombotest weniger ausgeprägt zu beobachten war. Diese Daten lassen vermuten, daß das Thrombotest-Verfahren sehr empfindlich ist gegen Kontakt- und Faktor-VII-Aktivierung und nicht gegen Coumarin-induzierte Inhibitoren.

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Author notes

  1. Alessandro Burul

    Present address: Institute of “Semeiotica Medica”, University of Padua Medical School, Italy

Authors and Affiliations

  1. Institute of “Semeiotica Medica”, University of Padua Medical School, Italy

    Antonio Girolami & Giovanni Patrassi

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  1. Antonio Girolami
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  2. Giovanni Patrassi
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  3. Alessandro Burul
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Additional information

This study was supported in part by a Grant from the “Anna Villa Rusconi Foundation”, Varese, and by a Grant from the C. N. R. (grant CT 75.00992.04), Rome.

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Girolami, A., Patrassi, G. & Burul, A. Decrease of Normotest/Thrombotest discrepancy in non-contacted coumarin plasma after glass or ellagic acid activation. Blut 33, 41–48 (1976). https://doi.org/10.1007/BF01005211

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