Skip to main content
Log in

Evidence for collagen molecular orientation in basement membranes

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Summary

The following basement membranes (BMs) from representative species of the main vertebrate classes were studied by the Picrosirius-polarization method: lens capsule, Reichert's membrane and glomerular BMs. A distinct birefringence was consistently observed in all BMs from all species studied by this method. The results reported provide a strong evidence for collagen macromolecular orientation in BMs. Heparitin sulphate was the only glycosaminoglycan detected in dog lens capsules.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Carlson, E. C., Brendel, K. &Meezan, E. (1978), Striated collagen fibrils derived from ultrastructurally pure isolated basal lamina. InBiology and Chemistry of Basement Membranes (edited byKefalides, N. A.), pp. 31–41. New York, San Francisco, London: Academic Press.

    Google Scholar 

  • Carlson, E. C., Meezan, E., Brendel, K. &Kenney, M. C. (1981) Ultrastructural analysis of control and enzyme-treated isolated renal basement membranes.Anat. Rec. 200, 421–36.

    Google Scholar 

  • Dehm, P. &Kefalides, N. A. (1978) The collagenous component of lens basement membrane.J. biol. Chem. 253, 6680–6.

    Google Scholar 

  • Farquhar, M. G. (1978), Structure and function in glomerular capillaries. InBiology and Chemistry of Basement Membranes (edited byKefalides, N. A.), pp. 43–80. New York, San Francisco, London: Academic Press.

    Google Scholar 

  • Farquhar, M. G., Wissig, S. L. &Palade, G. E. (1961), Glomerular permeability. I. Ferritin transfer across the normal glomerular capillary wall.J. exp. Med. 113, 47–66.

    Google Scholar 

  • Goldstein, D. J. (1969) Detection of dichroism with the microscope.J. Microsc. 89, 19–36.

    Google Scholar 

  • Hogan, M. J., Alvarado, J. A. &Weddell, J. E. (1971)Histology of the Human Eye, p. 653. Philadelphia, London, Toronto: Saunders.

    Google Scholar 

  • Junqueira, L. C. U., Bignolas, G. &Brentani, R. R. (1979b) Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections.Histochem. J. 11, 447–55.

    Google Scholar 

  • Junqueira, L. C. U., Bignolas, G. &Brentani, R. R. (1979b) A simple and sensitive method for the quantitative estimation of collagen.Analyt. Biochem. 94, 96–9.

    Google Scholar 

  • Junqueira, L. C. U., Bignolas, G., Mourào P. A. S. &Bonetti, S. S. (1980) Quantitation of collagen-proteoglycan interaction in tissue sections.Connect. Tiss. Res. 7, 91–6.

    Google Scholar 

  • Junqueira, L. C. U., Toledo, O. M. S. &Montes, G. S. (1981) Correlation of specific sulfated glycosaminoglycans with collagen types I, II, and III.Cell Tiss. Res. 217, 171–5.

    Google Scholar 

  • Kanwar, Y. S. &Farquhar, M. G. (1979) Isolation of glycosaminoglycans (heparan sulfate) from glomerular basement membranes.Proc. natn. Acad. Sci., U.S.A. 76, 4493–7.

    Google Scholar 

  • Kefalides, N. A. (1971), Isolation of a collagen from basement membranes containing three identical α-chains.Biochem. biophys. Res. Commun. 45, 226–34.

    Google Scholar 

  • Kefalides, N. A. (1978) Current status of chemistry and structure of basement membranes. InBiology and Chemistry of Basement Membranes (edited byKefalides, N. A.), pp. 215–28, New York, San Francisco, London: Academic Press.

    Google Scholar 

  • Kefalides, N. A., Alper, R. &Clark, C. C. (1979), Biochemistry and metabolism of basement membranes.Int. Rev. Cytol. 61, 167–228.

    Google Scholar 

  • Kefalides, N. A. &Winzler, R. J. (1966) The chemistry of glomerular basement membrane and its relation to collagen.Biochemistry 5, 702–13.

    Google Scholar 

  • Krakower, C. A. &Greenspon, S. A. (1978) The isolation of basement membranes. InBiology and Chemistry of Basement Membranes (edited byKefalides, N. A.), pp. 1–16. New York, San Francisco, London: Academic Press.

    Google Scholar 

  • Langeveld, J. (1981)Glomerular and tubular basement membranes, Thesis, University of Nijmegen.

  • Leivo, I., Vaheri, A., Timpl, R. &Wartiovaara, J. (1980) Appearance and distribution of collagens and laminin in the early mouse embryo.Devl Biol. 76, 100–14.

    Google Scholar 

  • McManus, J. F. A. (1946) Histological and histochemical uses of periodic acid.Stain Technol. 23, 99–108.

    Google Scholar 

  • Montes, G. S., Becerra, J., Toledo, O. M. S., Gordilho, M. A. &Junqueira, L. C. U. (1982) Fine structure and histochemistry of the tail fin ray in teleosts.Histochemistry 75, 363–76.

    Google Scholar 

  • Montes, G. S., Krisztán, R. M., Shigihara, K. M., Tokoro, R., Mourào, P. A. S. &Junqueira, L. C. U. (1980) Histochemical and morphological characterization of reticular fibers.Histochemistry 65, 131–41.

    Google Scholar 

  • Peters, K.-R., Kanwar, Y. S. &Farquhar, M. G. (1980) Fine structure of isolated glomerular basement membranes.J. Cell Biol. 87, 125a.

    Google Scholar 

  • Pierce, G. B. (1966) The development of basement membranes of the mouse embryo.Devl Biol. 13, 231–49.

    Google Scholar 

  • Pierce, G. B. &Dixon, F. J. (1959) Testicular teratomas. II. Teratocarcinoma as an ascitic tumor.Cancer 12, 584–9.

    Google Scholar 

  • Puchtler, H., Waldrop, F. S. &Valentine, L. S. (1973) Polarization microscopic studies of connective tissue stained with Picro-Sirius Red F3 BA.Beitr. Path. 150, 174–87.

    Google Scholar 

  • Roveri, N., Ripamonti, A., Bigi, A., Volpin, D. &Giro, M. G. (1979) X-ray diffraction study of bovine lens capsule collagen.Biochim. biophys. Acta,576, 404–8.

    Google Scholar 

  • Schwartz, D. &Veis, A. (1980) Characterization of bovine anterior-lens-capsule basement-membrane collagen.Eur. J. Biochem. 103, 29–37.

    Google Scholar 

  • Silva, M. E., Dietrich, C. P. &Nader, H. B. (1976) On the structure of heparitin sulfates. Analyses of the products formed from heparitin sulfates by two heparitinases and a heparinase fromFlavobacterium heparinum.Biochim. biophys. Acta 437, 129–41.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Junqueira, L.C.U., Montes, G.S., Toledo, O.M.S. et al. Evidence for collagen molecular orientation in basement membranes. Histochem J 15, 785–794 (1983). https://doi.org/10.1007/BF01003341

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01003341

Keywords

Navigation