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Functional and biochemical characteristics of a putative quisqualate-type receptor in rat striatum: Effect of brain lesions

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Abstract

Excitatory amino acids such asl-glutamate (Glu) and quisqualate (QUIS) markedly potentiated K+-evoked release of exogeneous [3H]dopamine (DA) from rat striatal slices. Intranstriatal kainic acid injections resulted in a total disappearance of the stimulatory effects of Glu on evoked-release of [3H]DA as well as in a parallel reduction in the maximal number (Bmax) of ad-aspartate-insensitivel-[3H]Glu binding site in striatal particulate fractions. Following cortical ablation, the potentiating effect of Glu on [3H]DA release in decorticated striatal slices lasted longer, compared to normal slices, and occured during the 2nd min following K+-depolarization. However, the extent (%) of Glu stimulation on [3H]DA release remained the same in decorticated and normal striatal slices. Cortical ablation produced also a significant decrease in the Bmax and in theK d′ of thed-aspartateinsensitive binding site towardsl[3H]Glu. These results support the proposal that thed-aspartate-insensitive Glu binding site is somehow related to an amino acid receptor-mediated modulation of dopaminergic transmission in the rat corpus striatum.

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Author notes

  1. Maria Isolde Rudolph

    Present address: Department of Physiological Sciences, Universidad de Concepción, Concepción, Chile

Authors and Affiliations

  1. Laboratory of Biochemical Pharmacology Department of Cell Biology, Pontificia Universidad Católica de Chile, Santiago, Chile

    Maria Isolde Rudolph & Gonzalo Bustos

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  1. Maria Isolde Rudolph
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  2. Gonzalo Bustos
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Isolde Rudolph, M., Bustos, G. Functional and biochemical characteristics of a putative quisqualate-type receptor in rat striatum: Effect of brain lesions. Neurochem Res 11, 1533–1545 (1986). https://doi.org/10.1007/BF00965772

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