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Uptake of piperidine and pipecolic acid by synaptosomes from mouse brain

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Abstract

Piperidine is actively transported into the synaptosomal fraction of adult mouse brain. The transport mechanism appears to be Na+ independent but is temperature dependent and sensitive to ouabain. Analysis of kinetic experiments indicates only a “low-affinity” transport system to be present. By contrast the uptake ofD,L-[3H]pipecolic acid at a concentration of 4×10−7 M was temperature and Na+ dependent, ouabain sensitive, and revealed a two-component system with aK m =3.9±0.17×10−6 M,V max=129±6 pmol/mg protein/3 min for the “high-affinity” system and aK m =90.2±4.3×10−6 M,V max=2.45±0.19 nmol/mg protein/3 min for the “low-affinity” system. Compounds structurally related to pipecolic acid such as glycine,l-proline, 4-amino-n-butyric acid, and 5-amino-n-valeric acid showed an inhibitory effect on uptake at a concentration of 10−4 M. The demonstration of biosynthesis of pipecolic acid in mouse brain and the presence of a “high-affinity” sodium-dependent uptake system suggest a physiological role of this substance in the central nervous system.

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Author notes

  1. Y. Nomura

    Present address: Department of Pharmacology, Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Kasumi 1-2-3, Hiroshima, Japan

  2. T. Schmidt-Glenewinkel

    Present address: Department of Genetics, Albert Einstein College of Medicine, 10641, Bronx, New York

Authors and Affiliations

  1. Laboratory of Neuropsychopharmacology Department of Biobehavioral Sciences, University of Connecticut, 06268, Storrs, Connecticut

    Y. Nomura, T. Schmidt-Glenewinkel & E. Giacobini

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  2. T. Schmidt-Glenewinkel
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  3. E. Giacobini
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Nomura, Y., Schmidt-Glenewinkel, T. & Giacobini, E. Uptake of piperidine and pipecolic acid by synaptosomes from mouse brain. Neurochem Res 5, 1163–1173 (1980). https://doi.org/10.1007/BF00964896

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