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The relationship between tegumental disruption and muscle contraction inSchistosoma mansoni exposed to various compounds

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Abstract

The antischistosomal drugs praziquantel and Ro 11-3128, which cause a tetanic contraction ofSchistosoma musculature, concurrently produce a disruption of the parasite's tegument. Both phenomena are attenuated by preincubation of the parasites in high Mg2+ containing media before addition of the drugs, and the therapeutically inactive stereoisomers of these drugs cause neither effect. Other antischistosomal drugs tested cause neither muscle contraction nor tegumental disruption. Some unrelated agents which cause muscle contraction (but have no antischistosomal effect) alter the parasite's tegument, while others do not. Cytochalasin B, but not colchicine, causes tegumental disruption without affecting muscle tension. Thus, there is no simple correlation between a drug's antischistosomal activity or ability to contract the parasite's muscle and its ability to damage the parasite's tegument.

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References

  • Becker B, Mehlhorn H, Andrews P, Thomas H, Eckert J (1980a) Light and electron microscopic studies on the effect of praziquantel onSchistosoma mansoni, Dicrocoelium dendriticum, andFasciola hepatica (Trematode) in vitro. Z Parasitenkd 63:113–128

    Google Scholar 

  • Becker B, Mehlhorn H, Andrews P, Thomas H (1980b) Scanning and transmission electron microscope studies on the efficacy of praziquantel onHymenolepis nana (Cestoda) in vitro. Z. Parasitenkd 61:121–133

    Google Scholar 

  • Becker B, Mehlhorn H, Andrews P, Thomas H (1981) Ultrastructural investigations on the effect of praziquantel on the tegument of five species of cestodes. Z Parasitenkd 64:257–269

    Google Scholar 

  • Bennett JL (1980) Characteristics of antischistosomal benzodiazepine binding sites inSchistosoma mansoni. J Parasitol 66:742–747

    Google Scholar 

  • Bogitsh BJ, Carter OS (1980)Schistosoma mansoni: Radioautography of colchicine's effect on3H-proline incorporation into adults in vitro. Exp Parasitol 49:319–327

    Google Scholar 

  • Chubb JM, Bennett JL, Akera T, Brody TM (1978) Effects of praziquantel, a new anthelmintic, on electromechanical properties of isolated rat atria. J Pharmacol Exp Ther 207:284–293

    Google Scholar 

  • Conder G, Marchiondo A, Anderson F (1981) Effect of praziquantel on adultEchinococcus granulosus in vitro: Scanning electron microscopy. Z. Parasitenkd 66:191–199

    Google Scholar 

  • Cornish RA, Bryant C (1976) Changes in energy metabolism due to anthelmintics inFasciola hepatica maintained invitro. Int J Parasit 6:393

    Google Scholar 

  • Cox FEG (1979) Death of a schistosome. Nature 278:401–402

    Google Scholar 

  • Fetterer RH, Pax RA, Bennett JL (1977)Schistosoma mansoni: Direct method for simultaneous recording of electrical and motor activity. Exp Parasitol 43:286–294

    Google Scholar 

  • Fetterer RH, Pax RA, Bennett JL (1980a) Praziquantel, potassium and 2,4-dinitrophenol: Analysis of their action on the musculature ofSchistosoma mansoni. Europ J Pharmacol 64:31–38

    Google Scholar 

  • Fetterer RH, Pax RA, Bennett JL (1980b)Schistosoma mansoni: Characterization of the electrical potential from the tegument of adult males. Exp Parasitol 49:353–365

    Google Scholar 

  • Fetterer RH, Pax RA, Bennett JL (1981) Na+−K+ transport, motility and tegumental membrane potential in adult maleSchistosoma mansoni. Parasitology 82:97–109

    Google Scholar 

  • Fetterer RH, Pax RA, Thompson D, Bricker C, Bennett JL (1980c) Praziquantel: Mode of its antischistosomal action. In: Van den Bossche (Hd) The host-invader interplay, Elsevier, Amsterdam, pp 695–698

    Google Scholar 

  • Jim K, Triggle DJ (1979) The actions of praziquantel and 1-methyladenine in guinea pig ileal longitudinal muscle. Can J Physiol Pharmacol 57:1460–1462

    Google Scholar 

  • Lee CM, Elhelu MA, Aboko-cole GF (1979) Immunobiology of schistosomiasis. J Nat Med Assoc 71:1191–1193

    Google Scholar 

  • Lin S, Chang Lin D, Flanagan MD (1979) Specificity of the effects of cytochalasin B on transport and motile processes. Proc Nat Acad Sci U.S. 75:329–333

    Google Scholar 

  • Mehlhorn H, Becker B, Andrews P, Thomas H, Frenkel JK (1981) In vivo and in vitro experiments on the effects of praziquantel onSchistosoma mansoni. Arzneim Forsch 31(I-3a):544–554

    Google Scholar 

  • Miller FH, Tulloch GS, Kuntz RE (1972) Scanning electron microscopy of integumental surface ofSchistosoma mansoni. J Parasitol 58:693–698

    Google Scholar 

  • Pappas PA, Read CP (1975) Membrane transport in helminth parasites: A review. Exp Parasitol 37:469–530

    Google Scholar 

  • Pax R, Bennett JL, Fetterer R (1978) A benzodiazepine derivative and praziquantel: Effects on musculature ofSchistosoma mansoni andSchistosoma japonicum. Naunyn-Schmiedeberg's Arch Pharmacol 304:309–315

    Google Scholar 

  • Pfeiffer DR, Reed PW, Lardy H (1974) Ultraviolet and fluorescent spectral properties of the divalent cation ionophore A-23187 and its metal ion complexes. Biochemistry 13:4007–4013

    Google Scholar 

  • Potter JD, Robertson SP, Johnson JD (1981) Magnesium and the regulation of muscle contraction. Federation Proc 40(12):2653–2656

    Google Scholar 

  • Silk MH, Spence IM, Buch B (1970) Observations ofSchistosoma mansoni blood flukes in the scanning electron microscope. S Afr J Med Sci 35:23–29

    Google Scholar 

  • Silk MH, Spence IM, Gear JHS (1969) Ultrastructural studies of the blood fluke:Schistosoma mansoni. I. The Integument. S Afr J Med Sci 34:1–10

    Google Scholar 

  • Voge M, Bueding F (1980)Schistosoma mansoni: tegumental surface alterations induced by subcurative doses of the schistosomicide amoscanate. Exp Parasitol 50:251–259

    Google Scholar 

  • Wessels NK, Spooner BS, Ash JF, Bradley MO, Luduena MA, Taylor EL, Wilson RA, Barnes PE (1974a) The tegument ofSchistosoma mansoni: Observations on the formation, structure and composition of cytoplasmic inclusions in relation to tegument function. Parasitology 68:239–258

    Google Scholar 

  • Wilson RA, Barnes PE (1974b) An in vitro investigation of dynamic processes occurring in the schistosome tegument, using compounds known to disrupt secretory processes. Parasitology 68:259–270

    Google Scholar 

  • Wilson RA, Barnes PE (1979) Synthesis of macromolecules by the epithelial surfaces ofSchistosoma mansoni: An autoradiographic study. Parasitology 78:295–310

    Google Scholar 

  • Wrenn JT, Yamada KM (1974) Microfilaments in cellular and developmental processes. Science 171:135–143

    Google Scholar 

  • Wolde Mussie E, Van de Waa J, Pax RA, Fetterer R, Bennett J (1982)Schistosoma mansoni: Calcium efflux and effects of calcium-free media on responses of the adult male musculature to praziquantel and other agents inducing contraction. Exp Parasitol 53:270–278

    Google Scholar 

  • Yuanquing Y, Huizhong Y, Shuhua X, Baoruo S, Xueming T, Jianguo Z, Huixin Z (1979) Observations on the histopathological changes ofSchistosoma japonicum and host liver caused by pyquiton in experimental chemotherapy. Acta Acad Med Sinicae 1:7–12

    Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology and Toxicology, and Neuroscience Program, Michigan State University, 48824, East Lansing, Michigan, USA

    C. S. Bricker, J. W. Depenbusch, J. L. Bennett & D. P. Thompson

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  1. C. S. Bricker
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  2. J. W. Depenbusch
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  3. J. L. Bennett
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  4. D. P. Thompson
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Bricker, C.S., Depenbusch, J.W., Bennett, J.L. et al. The relationship between tegumental disruption and muscle contraction inSchistosoma mansoni exposed to various compounds. Z. Parasitenkd. 69, 61–71 (1983). https://doi.org/10.1007/BF00934011

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