Abstract
The diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA) for antibodies toFasciola hepatica was evaluated using adult fluke excretory-secretory products as antigen. The sensitivity and specificity of the test were compared with those of the indirect hemagglutination (IHA) technique. The results of the DIG-ELISA showed that the reaction zone diameters obtained with 42 sera from sheep with natural or experimental exposure toF. hepatica were significantly greater than those obtained with 85 sera from ovines infected with other parasites. Both sensitivity and specificity were 100% in the DIG-ELISA, whereas the sensitivity was 68.2% and the specificity was 100% in the IHA. The data suggest that the DIG-ELISA is a valuable serodiagnostic test for sheep fascioliasis.
Similar content being viewed by others
References
Arriaga de Morilla C, Gómez A, Bautista CR, Morilla A (1988) Evaluación de un antígeno somático y uno metabólico deFasciola hepatica en el diagnóstico de la fasciolasis en ovinos. Tec Pecu Mex 26:203–211
Biagi F (1976) Enfermedades parasitarias. Segunda Edición. La Prensa Médica Mexicana, México
Burden DJ, Hammet NC (1978) Microplate enzyme linked immunosorbent assay for antibody toFasciola hepatica in cattle. Vet Record 103:158
Castilla MM, Santos-Gómez M, Guzmán-Bracho C, Bautista-Garfias CR (1988) A new method for diagnosis of Chagas' disease: Diffusion-in-gel enzyme-linked immunosorbent assay. J Parasitol 74:805–809
Cornish RA, Brvant C (1976) The metabolic integrity ofFasciola hepatica during in vitro maintenance. Int J Parasitol 6:387–392
Dawes B (1954) Maintenance in vitro ofFasciola hepatica. Nature 174:654–655
Elwing H, Nygren H (1979) Diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA): A simple method for quantitation of class-specific antibodies. J Immunol Methods 31:101–107
Elwing H, Lange S, Nygren H (1980) Diffusion-in-gel enzymelinked immunosorbent assay (DIG-ELISA): Optimal conditions for quantitation of antibodies. J Immunol Methods 39:247–256
Espino AN, Duménigo BE, Fernández R, Finlay CM (1987) Immunodiagnosis of human fascioliasis by enzyme-liked immunosorbent assay using excretory-secretory products. Am J Trop Med Hyg 37:605–608
Gómez-Priego A, Paniagua-Solis JF, Rivas-Alcalá R, Ruenes-Meza MT Successful application of the diffusion-ingel enzyme-linked immunosorbent assay (DIG-ELISA) in onchocerciasis. Trans Roy Soc Trop Med Hyg 79:159–161
Grelck H, Hörchner F (1977) Comparative studies on the serodiagnosis of bovine fascioliasis with the indirect immunofluorescence test and the immunoperoxidase test (ELISA). Berl Münch Tierärztl Wochenschr 90:332–335
Kagan IG (1980) Other trematodes and cestodes. In: Houba V (ed) Immunological investigation of tropical parasitic diseases. Churchill Livingstone, Edinburgh, pp 148–156
Kagan IG, Norman L (1976) Serodiagnosis of parasitic diseases. In: Rose N, Friedman H (eds) Manual of Clinical Immunology. American Society for Microbiology, Washington DC, pp 382–409
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Quiroz H (1984) Parasitologia y enfermedades parasitarias de animales domésticos. LIMUSA, México
Tizard I (1982) Serologic assays. J Am Vet Med Assoc 181:1162–1165
Uggla A, Nilsson L-Å (1987) Evaluation of a solid-phase immunoassay (DIG-ELISA) for the serodiagnosis of ovine toxoplasmosis. Vet Immunol Immunopathol 14:309–318
Wescott RB, Farrell CJ, Shen DT (1984) Diagnosis of naturally occurringFasciola hepatica infections in cattle with an enzyme-linked immunosorbent assay. Am J Vet Res 45:178–179
Zimmerman GL, Jen LW, Cerro JE, Farnsworth KL, Wescott RB (1982) Diagnosis ofFasciola hepatica infections in sheep by an enzyme-linked immunosorbent assay. Am J Vet Res 43:2097–2100
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bautista-Garfias, C.R., López-Arellano, M.E. & Sánchez-Albarrán, A. A new method for serodiagnosis of sheep fascioliasis using helminth excretory-secretory products. Parasitol Res 76, 135–137 (1989). https://doi.org/10.1007/BF00930835
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00930835