Summary
A method of blood preparation is described which resulted in the successful rearing ofGlossina p. palpalis with in vitro feeding. Cells of defibrinated bovine blood were washed in 0.89% NaCl solution prior to deep-freezing. Cells and serum were stored (−28°C) for up to four months. For feeding, both components were mixed in the volumetric proportion 1:1. Adenosine triphosphate was added at a concentration of 10−3 M to stimulate uptake of blood. Survival rate and mean weight of puparia remained constant over three generations while productivity increased.
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Galun, R., Margalit, J.: Adenosine nucleotides as feeding stimulants of the tsetse fly,Glossina austeni Newst. Nature22, 583–584 (1969)
Galun, R., Rice, M.J.: Role of blood platelets in haematophagy. Nature New Biol.233, 110–111 (1972)
Goodger, B.V.: Fibrinogen-like proteins and fibrinolytic proteins in saline eluate from sucrose washed bonine erythrocytes. Aust. J. Exp. Biol. Med. Sci.54, 381–390 (1976)
Graf, U., Henning, H.J., Stange, K.: Formeln und Tabellen der mathematischen Statistik, pp. 75–76. Berlin, Heidelberg, New York: Springer 1966
Itard, J., Jordan, A.M.: Mass rearing using animals for feeding. In: Tsetse: The future for biological methods in integrated control, M. Laird, ed., pp. 124–140. Ottawa: IDRC 1977
Langley, P.A.: The role of physical and chemical stimuli in the development of in vitro feeding technique for tsetse flies,Glossina spp. (Diptera, Glossinidae). Bull. Entomol. Res.62, 215–228 (1972)
Langley, P.A., Pimley, R.W.: Quantitative aspects of reproduction and larval nutrition inGlossina morsitans Westw. (Diptera, Glossinidae) fed in vitro. Bull. Entomol. Res.65, 129–142 (1975)
Mews, A.R.: The laboratory colonization ofGlossina morsitans morsitans Westw. fed through membranes with rabbit supplement. Rev. Elev. Med. Vet. Pays Trop., Suppl., 57–59 (1974)
Mews, A.R., Baumgartner, H., Luger, D., Offori, E.D.: Colonisation ofGlossina morsitans morsitans Westw. in the laboratory using in vitro feeding techniques. Bull. Entomol. Res.65, 631–641 (1976)
Mitchell, B.K.: Physiology of an ATP receptor in labellar sensilla of the tsetse flyGlossina m. morsitans (Diptera, Glossinidae). J. Exp. Biol.65, 259–271 (1976)
Wetzel, H.: Mass production using in vitro feeding. In: Tsetse: The future for biological methods in intergrated control, M. Laird, ed., pp. 141–148, Ottawa, IDRC 1977
Wetzel, H.: Comparison of colony performance and generation sequence in the rearing ofGlossina p. palpalis (Rob.-Desv.) using in vitro feeding (Diptera, Glossinidae). Z. Angew. Zool. (in press, 1978)
Wetzel, H., Luger, D.: In vitro feeding in the rearing of tsetse flies (Glossina m. morsitans andG. p. palpalis, Diptera: Glossinidae). Tropenmed. Parasitol.29, 239–251 (1978)
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Wetzel, H., Luger, D. The use of deep frozen, stored bovine blood for in vitro feeding of tsetse flies. Z. Parasitenkd. 57, 163–168 (1978). https://doi.org/10.1007/BF00927157
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DOI: https://doi.org/10.1007/BF00927157