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Cellular changes in bone marrow of malaria-infected mice

III. Chemotaxis of Granulocytes

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Abstract

The number of bone marrow cells and their chemotactic activity was studied during malaria infection. Two days after infection of Balb/c mice withPlasmodium berghei, an increase in granulocyte number was observed in the blood. A modified Boyden chamber chemotaxis assay was employed to investigate the mechanism of granulocyte accumulation in the blood. Bone marrow cells from normal mice, from mice during a primary lethal infection and from immune mice after challenge were compared. The complement factor C5a showed chemotactic activity for bone marrow cells; a significant decrease of chemotaxis was only observed after 6 days of primary infection. Extracts of spleen, liver and infected erythrocytes lacked chemotactic activity, or caused inhibition of cell migration. Serum from mice with a 2-day primary infection contained chemotactic activity. The active component was heat labile, protease sensitive and had an estimated molecular weight of 250,000.

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Authors and Affiliations

  1. Department of Protozoology, The Hebrew University-Hadassah Medical School, PO 1172, Jerusalem, Israel

    S. Frankenburg, M. V. Londner & C. L. Greenblatt

  2. S.F. Kuvin Centre for the Study of Infectious and Tropical Diseases, The Hebrew University-Hadassah Medical School, PO 1172, Jerusalem, Israel

    S. Frankenburg & M. V. Londner

Authors
  1. S. Frankenburg
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  2. M. V. Londner
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  3. C. L. Greenblatt
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Frankenburg, S., Londner, M.V. & Greenblatt, C.L. Cellular changes in bone marrow of malaria-infected mice. Z. Parasitenkd. 68, 39–45 (1982). https://doi.org/10.1007/BF00926656

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  • DOI: https://doi.org/10.1007/BF00926656

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