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Expression analysis of the mixed function oxidase system in rat brain by the polymerase chain reaction

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Abstract

Metabolism of therapeutic drugs in the body by the mixed function oxidase system is an important consideration in the analysis of a drug's effectiveness. P450-dependent metabolism within the brain of a neuro-specific drug may affect the drug's course of action. To determine whether cytochrome P450 was expressed in brain, RNA was isolated from the whole brains of rats treated with a variety of known hepatic P450 inducers, including amitriptyline, imipramine, isosafrole, phenobarbital, and β-naphthoflavone. The RNA was analyzed for the presence of P450 isozymes by the PCR technique. Differential expression of P450IA1, P450IIB1, P450IIB2, P450IID, and P450IIE1 was detected in the brain samples, depending on the treatment. Cytochrome P450 reductase expression was also detected in the brain samples, giving strong evidence that the brain contains a competent mixed function oxidase system under all conditions studied. (Mol Cell Biochem120: 171–179, 1993)

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Author notes

  1. Terry B. White

    Present address: Lab 1, Central Receiving, Evergreen State College, 2700 Evergreen College Parkway, 98505, Olympia, WA, USA

  2. James W. White

    Present address: The Washington State Department of Health, Office of Toxic Substances, 98504, Olympia, WA, USA

Authors and Affiliations

  1. The Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston, P.O. Box 20708, 77225, Houston, Texas, USA

    Anne V. Hodgson, Terry B. White, James W. White & Henry W. Strobel

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  1. Anne V. Hodgson
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  2. Terry B. White
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  3. James W. White
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  4. Henry W. Strobel
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Thesis student of the Graduate School of Biomedical Sciences, the University of Texas Health Science Center at Houston

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Hodgson, A.V., White, T.B., White, J.W. et al. Expression analysis of the mixed function oxidase system in rat brain by the polymerase chain reaction. Mol Cell Biochem 120, 171–179 (1993). https://doi.org/10.1007/BF00926090

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  • DOI: https://doi.org/10.1007/BF00926090

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