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In vitro propagation of herbaceous peony (paeonia lactiflora pall.) by a longitudinal shoot-split method

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Abstract

A procedure for the clonal propagation ofPaeonia lactiflora Pall. cvs. Takinoyosooi and Sarah Bernhardt through shoot tip culture is described. Half strength Murashige and Shoog (1962) medium supplemented with 0.5 mg/l 6-benzylaminopurine plus 1 mg/l gibberellic acid promoted formation and growth of axillary buds. Continuous shoot multiplication was achieved by vertically splitting the shoot axis and subsequent division of elongated axillary shoots every 36 days. High frequency (57–100%) of rooting was obtained on paper-bridge liquid medium supplemented with 1 mg/l indole-3-butyric acid. Half of the rooted plantlets were established on porous soil. Thus, 700 and 300 plants of cv. Takinoyosooi and Sarah Bernhardt could be theoretically obtained from a single bud in one year.

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Abbreviations

BAP:

6-benzylaminopurine

GA:

gibberellic acid

NAA:

a-naphthaleneacetic acid

IBA:

indole-3-butyric acid

MS:

Murashige and Skoog (1962) basal medium

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Authors and Affiliations

  1. Laboratory of Vegetable and Ornamental Horticulture, Faculty of Agriculture, Shimane University, Nishikawatsu-cho 1060, 690, Matsue, Japan

    Takashi Hosoki, Michiko Ando, Takehito Kubara, Morihiko Hamada & Masato Itami

Authors
  1. Takashi Hosoki
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  2. Michiko Ando
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  3. Takehito Kubara
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  4. Morihiko Hamada
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  5. Masato Itami
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Communicated by M. Tabata

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Hosoki, T., Ando, M., Kubara, T. et al. In vitro propagation of herbaceous peony (paeonia lactiflora pall.) by a longitudinal shoot-split method. Plant Cell Reports 8, 243–246 (1989). https://doi.org/10.1007/BF00778543

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  • DOI: https://doi.org/10.1007/BF00778543

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