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Evaluation of several enrichment procedures for the isolation of recombinant plasmid DNA

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Abstract

A number of methods for the selective enrichment of recombinant plasmids were examined; these include alkaline phosphatase treatment of the restricted pBR322 vector, as well as a combination of this and S1 nuclease treatment of the ligated mixture of pBR322 and pCR1 plasmids orS. griseus DNA followed by D-cycloserine treatment to enrich for cells carrying recombinant molecules. The relative efficiencies of these methods were compared.

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Authors and Affiliations

  1. Department of Virology, Weizmann Institute of Science, Rehovot, Israel

    Arie Rosner, Roberto N. Bastos & Haim Aviv

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  1. Arie Rosner
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  2. Roberto N. Bastos
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  3. Haim Aviv
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Rosner, A., Bastos, R.N. & Aviv, H. Evaluation of several enrichment procedures for the isolation of recombinant plasmid DNA. Mol Biol Rep 4, 253–256 (1979). https://doi.org/10.1007/BF00777564

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  • DOI: https://doi.org/10.1007/BF00777564

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