Summary
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1.
Fluoride and peptide-stimulated adenylate cyclase activity was investigated by electron histochemistry on serial sections of the RPAI neuron of the snailHelix pomatia.
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2.
Fluoride-stimulated adenylate cyclase was detected in the surface membrane of the RPAI neuron, the postsynaptic membrane of axosomatic contacts, and the surface of glial cells forming a multilayer capsule around the neuron.
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3.
Peptide-stimulated adenylate cyclase was located in the membrane of glial cells surrounding the neuron, their processes (trophospongia) invaginating deeply in the neuronal soma, and the membrane of somatic protrusions forming the system of lacoons in the region of the axosomatic contact.
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4.
No peptide-stimulated adenylate cyclase was revealed in the remaining part of the surface of the somatic membrane.
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5.
The localization of adenylate cyclase activity in the postsynaptic membrane in the region of the axosomatic contact is in accordance with the hypothesis based on electrophysiological experiments that the cyclase system participates in the genesis and regulation of the bursting activity of the RPAI neuron.
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References
Cutler, L. S., and Christian, C. P. (1980). Cytochemical localization of adenylate cyclase.J. Histochem. Cytochem. 2862–65.
Elekes, K., Vadasz, I., and Salanki, J. (1979). Ultrastructure of the bimodal pacemaker neuron in the central nervous system ofHelix pomatia L.Acta Biol. Acad. Sci. Hung. 30317–334.
Howell, S., and Whitfield, M. (1972). Cytochemical localization of adenylate cyclase activity in rat islets of Langerhans.J. Histochem. 20873–879.
Ifshin, M. S., Gainer, H., and Barker, J. L. (1975). Peptide factor extracted from molluscan ganglia that modulates bursting pacemaker activity.Nature 25472–74.
Kempen, H. J. M., dePont, J. J. H. H. M., Bonting, S. L., and Stadhouders (1978). The cytochemical localization of adenylate cyclase: Fact or artifact?J Histochem. Cytochem. 26298–312.
Kononenko, N. I. (1979a). Modulation of the endogenous electrical activity of the bursting neuron in the snailHelix pomatia. I. The generator of the slow rhythms.Neuroscience 122037–2045.
Kononenko, N. I. (1979b). Modulation of the endogenous electrical activity of the bursting neuron in the snailHelix pomatia. II. The membrane characteristics related to modulation of the endogenous activity of the neuron.Neuroscience 122047–2054.
Kononenko, N. I. (1979c). Modulation of the endogenous electrical activity of the bursting neuron in the snailHelix pomatia. III. A factor modulating the endogenous electrical activity of the bursting neuron.Neuroscience 122055–2060.
Kononenko, N. I. (1980). The effect of application of the factor modulating the electrical activity in the soma of the snailHelix pomatia bursting neuron.Dokl. Akad. Nauk USSR 2501490–1493.
Kononenko, N. I. (1981a). Effect of theophylline on the electrical activity of the bursting neuron inHelix pomatia.Neurophysiology 1375–79 (Russian).
Kononenko, N. I. (1981b). Transitions of electrical activity in the RPAI neuron of the snailHelix pomatia.Neurophysiology 13398–405 (Russian).
Koval, L. M., Kononenko, N. I., and Skibo, G. G. (1982). Axosomatic contacts on the bursting neuron of the snailHelix pomatia and histochemical detection of the adenylate cyclase activity in them.Neurophysiology 14435–437. (Russian).
Koval, L. M., Kononenko, N. I., and Skibo, G. G. (1984). The axosomatic contacts on the bursting neuron of the snailHelix pomatia. I. Ultrastructural features of the axosomatic contacts.Cell. Mol. Neurobiol. 431–41.
Lemay, and Jarett, L. (1975). Pitfalls in the use of lead nitrate for the histochemical demonstration of adenylate cyclase activity.J. Cell. Biol. 6539–44.
Levitan, I., and Treistman, S. (1977). Modulation of electrical activity and cyclic nucleotide metabolism in molluscan nervous system by a peptide-containing nervous system extract.Brain Res. 136307–317.
Levitan, I. B. (1978a). Adenylate cyclase in isolatedHelix andAplysia neuronal cell bodies: Stimulation by serotonin and peptide-containing extract.Brain Res. 154404–408.
Levitan, I. B., (1978b). Modulation of neuronal activity by peptides and neurotransmitters: Possible role of cyclic nucleotides.J. Physiol. 74521–526.
Levitan, I. B., Bergstroem, E., and Simonet, M. (1978). Adenylate cyclase inHelix andAplysia ganglia: Characteristics of its stimulation by a peptide-containing nervous system extract.J. Neurochem. 311353–1359.
Reik, J., Petzold, G. L., Higgins, J. A., Greengard, P., and Barrnett, R. (1970). Hormone-sensitive adenyl cyclase: Cytochemical localization in rat liver.Science 168382–384.
Schulze, W., Hinterberger, U., and Wollenberger, A. (1978). Cytochemistry of adenylate cyclase. Problems and practice. InBiophysical and Biochemical Information Transfer in Recognition (Vassileva-Popova, J. C., and Jensen, V., Eds.), Plenum, New York, p. 341.
Trandabaru, T. (1976). Ultrastructural localization of adenyl cyclase activity in the pancreas of two amphibian species (Salamandra salamandra L. andRana esculenta L.)Histochemistry 481–5.
Treistman, S. N., and Levitan, Y. B. (1976). Alteration of electrical activity in molluscan neurones by cyclic nucleotides and peptide factors.Nature 26162–64.
Tsuchiya, T., and Tamate, H. (1979). Ultrastructural localization of adenyl cyclase activity in sheep parathyroid gland.Acta Histochem. Cytochem. 12356–360.
Wagner, R. C., and Bitensky, M. W. (1974). Adenylate cyclase in electron microscopy of enzymes. InPrinciples and Methods (Hayat, M. A., Ed.,), Van Nostrand, New York, Vol. 2, pp. 110–131.
Wollenberger, A., and Schulze, W. (1976). Cytochemical studies on sarcolemma: Na+, K+-adenosine triphosphatase and adenylate cyclase. InRecent Advances in Studies on Cardiac Structure and Metabolism (Roy, P. E., and Dhalla, N. S., Eds.), University Park Press, Baltimore, Vol. 9, p. 101.
Yount, R. G., Badcock, D., Ballantyne, W., and Ojala, D. (1971). Adenylylimidodiphosphate, an adenosine triphosphate analog containing a PNP linkage.Biochemistry 102484–2486.
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Koval, L.M., Kononenko, N.I. & Skibo, G.G. The axosomatic contacts on the bursting neuron of the snailHelix pomatia. II. ultrastructural localization of adenylate cyclase. Cell Mol Neurobiol 4, 43–52 (1984). https://doi.org/10.1007/BF00710941
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DOI: https://doi.org/10.1007/BF00710941