Summary
Dendritic growth and dendritic arborization of both the large neurons of the cerebral and the cerebellar cortex and the small bipolar neurons were studied in vitro under normal feeding conditions and under the influence of GABA, glycine, and sodium barbiturate.
By the end of week 1 the neurons cultured in normal nutrient developed primary dendritic shafts, demonstrating a tendency for bifurcation. By the end of week 2 the neurons appeared as numerous secondary dendritic branches studded with spines. The dendritic development and growth proceeded continuously until week 12 when no further growth and differentiation of the dendritic arborization was noted.
Feeding medium enriched with GABA or glycine enhanced dendritic growth and dendritic arborization in vitro. On the contrary, feeding medium contained sodium barbiturate, partially suppressed dendritic growth and dendritic arborization in the neurons of the cerebral and the cerebellar explants. Ultrastructural studies revealed that sodium barbiturate partially suppressed the synapse formation between the neuronal circuits of the cortical explants.
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References
Achar VC, Welch KMA, Chabi E (1976) Cerebrospinal fluid γ-aminobutyric acid in neurologic diseases. Neurology 26:777–780
Aprison MH, Davidoff RA, Werman R (1970) Glycine: its metabolism and possible transmitter roles in nervous tissue. In: Lajtha A (ed) Handbook of neurochemistry, vol 3. Plenum Press, New York, p 381
Bala Manyam NV, Katz L, Hare T, Gerber T, Grossman M (1980) Levels of γ-aminobutyric acid in cerebrospinal fluid in various neurologic disorders. Arch Neurol 37:352–355
Baloyannis SJ, Kim SU (1979) Experimental modification of cerebellar development in tissue culture: X-irradiation induces granular degeneration and unattached Purkinje cell dendritic spines. Neurosci Lett 12:284–288
Baxter CF (1970) The nature of γ-aminobutyric acid. In: Lajtha A (ed) Handbook of neurochemistry, vol 3. Plenum Press, New York, p 289
Bazemore AW, Elliott KAC, Florey E (1957) Isolation of factor I. J Neurosci 1:334–339
Bird ED, Iversen LL (1974) Huntington's chorea: postmortem measurement of glutamic acid decarboxylase, cholin acetylase and dopamine in basal ganglia. Brain 97:457–472
Curtis DR, Hosli L, Johnston GAR (1968) The hyperpolarization of spinal motoneurons by glycine and related amino acids. Exp Brain Res 5:235–258
Curtis DR, Duggan A, Johnston GAR (1970) The inactivation of extracellularly administered amino acids in the feline spinal cord. Exp Brain Res 10:447
Curtis DR, Felix D (1971) GABA and prolonged spinal inhibition. Nature 231:187–188
Godfraind J, Kawamura H, Krnjevic K, Pumain R (1971) Actions of dinitrophenol and some other metabolic inhibitors on cortical neurons. J Physiol 215:199–222
Davidoff RA (1972) Gamma aminobutyric acid antagonism and presynaptic inhibition in the frog spinal cord. Science 175:331–333
Hamori J (1973) Developmental morphology of dendritic postsynaptic specialization. In: Lissak K (ed) Recent developments of neurobiology in Hungary. Results in neuroanatomy, neuroendocrinology, neurophysiology and behavior physiology, vol 4. Akademiai Kiado, Budapest, pp 19–32
Harrison RG (1907) The outgrowth of the nerve fibers as a model of protoplasmic movement. J Exp Zool 9:787
Hayashi T (1954) Effects of sodium glutamate on the nervous system. Keia J Med 3:183–192
Herdon RM (1968) Thiopen induced granule cell necrosis in the rat cerebellum. An electron microscopic study. Exp Brain Res 6:49–68
Hinds JW, Hinds PL (1972) Reconstruction of dendritic growth cones in neonatal mouse olfactory bulb. J Neurocytol 1:169–187
Kim SU, Pazeshkpour G (1975) Unattached Purkinje cell dendritic spines in cytosine arabinoside treated mice. J Neuropathol Exp Neurol 35:112
Krnjevic K (1972) Excitable membranes and anesthetics in cellular biology and toxicity of anesthetics. Fink BR (ed) Williams and Wilkins. Baltimore, pp 3–9
Maurien-Mathieu AM, Colonnier M (1969) The molecular layer of the adult cat cerebellar cortex after lesions of the parallel fibers: An optic and electron microscopic study. Brain Res 16: 307–323
Morest DK (1969) The growth of the dendrite in mammalian brain. Z Anat Entwickl Gesch 128:290–317
Otsuka M, Miyata Y (1972) Application of enzymatic cycling to the measurement of gamma aminobutyric acid in single neurons of the mammalian central nervous system. In: Costa E, Iversen I, Paoletti R (eds) Studies of neurotransmitters of the synaptic level. Raven Press, New York, pp 61–74
Privat A (1975) Dendrilic growth in vitro. In: Kreutzberg GW (ed) Advances in neurology, vol 12. Raven Press, New York, pp 201–216
Richards CD (1972) On the metabolism of barbiturate anesthesia. J Physiol (Lond) 227:749–767
Robinson N, Wells F (1973) Distribution and localization of sites of gamma aminobutyric acid metabolism in the adult brain. J Anat 114:365–378
Seeman P (1972) The membrane actions of anesthetics and tranquilizers. Pharmacol Rev 24:583
Shankaran R, Quastel JM (1972) Effects of anesthetics on sodium uptake into rat brain cortex in vitro. Biochem Pharmacol 21: 1763–1773
Sotelo C, Palay SL (1968) The fine structure of the lateral vestibular nucleus in the rat. I: Neurons and neurologlial cells. J Cell Biol 36:151–174
Tapia R, Sandoval ME (1974) Possible participation of γ-aminobutyric acid in the regulation of protein synthesis in the brain, in vitro. Brain Res 69:255–263
Vaughn JE, Henrikson CR, Grieshabar JA (1974) A quantitative study of synapses in motor neuron dendritic growth cones in developing spinal cord. J Cell Biol 60:664–672
Weakly JN (1969) Effects of barbiturates on “quantal” synaptic transmission in spinal motorneurons. J Physiol (Lond) 204: 63–77
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Baloyannis, S.J., Karakatsanis, K., Karathanasis, J. et al. Effects of GABA, glycine, and sodium barbiturate on dendritic growth in vitro. Acta Neuropathol 59, 171–182 (1983). https://doi.org/10.1007/BF00703201
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DOI: https://doi.org/10.1007/BF00703201