Summary
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1.
The effects of a decrease of the K+ gradient on the extraneuronal inward transport and outward movement of catecholamines were studied in rat heart, rabbit aortic rings and guinea-pig trachealis smooth muscle. Elevation of the extracellular K+ concentration caused a) inhibition of the corticosteroid-sensitive extraneuronal uptake (uptake2) of3H-isoprenaline in rat heart and of3H-noradrenaline in rabbit aorta, and b) acceleration of efflux of3H-isoprenaline from rat heart,3H-noradrenaline from rabbit aorta and adrenaline (measured by microphotometry) from guinea-pig trachealis muscle.
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2.
In rat heart and rabbit aorta, the acute omission of one or the other of the ions Na+, Cl−, K+ or Ca2+ from the perfusion or incubation medium had no effect on initial rates of uptake2 of catecholamines, except that the absence of K+ had a small inhibitory effect in the rat heart.
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3.
The prolonged absence of Na+, Ca2+ or K+ from the perfusion or incubation medium caused a marked inhibition of uptake2 of catecholamines. These inhibitory effects developed more quickly in rat heart than in rabbit aorta.
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4.
These results are compatible with the possibility that either the K+ gradient across the cell membrane or the resting membrane potential is the force driving uptake2.
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Some of the results were communicated to the German Pharmacological Society (Bönisch et al 1982). This study was supported by the Deutsche Forschungsgemeinschaft and the National Health and Medical Research Council of Australia
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Bönisch, H., Bryan, L.J., Henseling, M. et al. The effect of various ions on uptake2 of catecholamines. Naunyn-Schmiedeberg's Arch. Pharmacol. 328, 407–416 (1985). https://doi.org/10.1007/BF00692909
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DOI: https://doi.org/10.1007/BF00692909