Abstract
Pseudomonas sp. B13 was grown in continuous culture on 4-chlorophenol as the only carbon source. Maximum growth rate of 0.4h-1 was observed at a substrate concentration of >0.01 mM and <0.15 mM. In addition to the enzymes of phenol catabolism, high specific 1,2-dioxygenase activities with chlorocatechols as substrates were found. The isomeric monochlorinated phenols were also totally degraded by 4-chlorophenol grown cells. (+)-2,5-Dihydro-4-methyl- and (+)-2,5-dihydro-2-methyl-5-oxo-furan-2-acetic acid were formed in high yield as dead-end catabolites from cooxidation of cresoles.
Several dichlorophenols except 2,6-dichlorophenol were removed from the culture fluid by chlorophenol grown cells. Ring cleavage of chlorinated catechols were shown to be one of the critical steps in chlorophenol catabolism. A catabolic pathway for isomeric chlorophenols is discussed.
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Abbreviations
- HPLC:
-
High performance liquid chromatography
- DHB:
-
“Dihydrodihydroxybenzoate” 3,5-cyclohexadiene-1,2-diol-1-carboxylic acid
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Knackmuss, HJ., Hellwig, M. Utilization and cooxidation of chlorinated phenols byPseudomonas sp. B 13. Arch. Microbiol. 117, 1–7 (1978). https://doi.org/10.1007/BF00689343
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DOI: https://doi.org/10.1007/BF00689343