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Induction of epitopes associated with neurofibrillary tangles in clonal mouse neuroblastoma (S20Y) cells

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Summary

Accumulation of paired helical filaments (PHF) in neurofibrillary tangles is a key neuropathological hallmark in Alzheimer's disease (AD). To date, PHF have been found primarily in humans. Cultured murine cholinergic neuroblastoma (S20Y) cells, following exposure to a serum-free medium or a differentiation medium, developed immunoreactivity to anti-PHF antibodies, and to the Alz-50 by immunocytochemical and immunoblot analyses. Electron microscopic examination revealed abundant fascicles of 10-nm filaments coursing tortuously amongst organelles, such as mitochondria, endoplasmic reticulum and dense-core vesicles, in perikarya and in neuritic extensions. However, subcellular structures identical or similar to PHF could not be found in these non-human cells. This convenient cell culture model may prove to be useful for studying certain aspects of the mechanisms underlying the abnormal cytoskeletal alterations which are characteristic of AD and related neurodegenerative disorders.

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Authors and Affiliations

  1. Altschul Laboratory for Dementia Research, Cornell University Medical College, Burke Rehabilitation Center, 10605, White Plains, NY, USA

    L. Ko, K. -F. R. Sheu, O. Young & J. P. Blass

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  1. L. Ko
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  2. K. -F. R. Sheu
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  3. O. Young
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  4. J. P. Blass
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Supported by grants from the Overbrook Foundation, the Will Rogers Institute, the Dr. I. Fund Foundation, the Winifred Masterson Burke Relief Foundation, the Alzheimer's Disease Research Program of the American Health Assistance Foundation and the National Institute of Aging (AG03853)

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Ko, L., Sheu, K.F.R., Young, O. et al. Induction of epitopes associated with neurofibrillary tangles in clonal mouse neuroblastoma (S20Y) cells. Acta Neuropathol 81, 30–40 (1990). https://doi.org/10.1007/BF00662635

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  • DOI: https://doi.org/10.1007/BF00662635

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