Abstract
Cardiac muscle fragments with disrupted sarcolemmas were prepared by homogenization of mouse ventricles. The rate of spontaneous contractions was increased when a solution containing isobutyrate as the main anion was substituted with a solution rich in chloride. At low calcium concentrations preparations which were quiescent in the isobutyrate solution responded to the chloride with a strong single contraction.
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Best, P. M., Donaldson, S. K. B., Kerrick, W. G. L.: Tension in mechanically disrupted mammalian cardiac cells: Effects of magnesium adenosine triphosphate. J. Physiol. (Lond.)265, 1–17 (1977)
Costantin, L. L., Podolsky, R. J.: Depolarization of the internal membrane system in the activation of frog skeletal muscle. J. Gen. Physiol.50, 1101–1124 (1967)
Endo, M.: Calcium release from the sarcoplasmic reticulum. Physiol. Rev.57, 71–108 (1977).
Endo, M., Blinks, J. R.: Inconstant association of aequorin luminescence with tension during calcium release in skinned muscle fibres. Nature New Biol.246, 218–220 (1973)
Fabiato, A., Fabiato, F.: Excitation-contraction coupling of isolated cardiac fibers with disrupted or closed sarcolemmas. Circ. Res.31, 293–307 (1972)
Fabiato, A., Fabiato, F.: Techniques of skinned cardiac cells and of isolated cardiac fibers with disrupted sarcolemmas with reference to the effects of catecholamines and of caffeine. In: Recent advances in studies on cardiac structure and metabolism, Vol. 9, The sarcolemma (P. E. Roy and N. S. Dhalla, eds.), pp. 71–94. Baltimore: University Park Press 1975
Fabiato, A., Fabiato, F.: Calcium release from the sarcoplasmic reticulum. Circ. Res.40, 119–129 (1977a)
Fabiato, A., Fabiato, F.: Variations of the membrane potential of the sarcoplasmic reticulum of skinned cells from cardiac and skeletal muscle detected with a potential sensitive dye. J. Gen. Physiol.70, 6a (1977b)
Kasai, M., Miyamoto, H.: Depolarization-induced calcium release from sarcoplasmic reticulum fragments. I. Release of calcium taken up upon using ATP. J. Biochem.79, 1053–1066 (1976)
Kerrick, W. G. L., Best, P. M.: Calcium ion release in mechanically disrupted heart cells. Science183, 435–437 (1974)
Melchior, N. C.: Sodium and potassium complexes of adenosine triphosphate: equilibrium studies. J. Biol. Chem.208, 615–627 (1954)
Nakajima, Y., Endo, M.: Release of calcium induced by “depolarisation” of the sarcoplasmic reticulum membrane. Nature New Biol.246, 216–218 (1973)
Nanninga, L. B.: The association constant of the complexes of adenosine triphosphate with magnesium, calcium, strontium, and barium ions. Biochim. Biophys. Acta54, 330–338 (1961)
Schwarzenbach, G., Senn, H., Anderegg, G.: Komplexone XXIX. Ein großer Chelateffekt besonderer Art. Helv. Chim. Acta40, 1886–1900 (1957)
Sillén, L. G., Martell, A. E.: Stability constants of metal-ion complexes. London: The Chemical Society 1964
Trube, G.: Contractions of skinned cardiac cells elicited bycurrent pulses. Pflügers Arch.376, 217–222 (1978)
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This work was supported by the Deutsche Forschungsgemeinschaft, SFB 38 Membranforschung, Projekt G
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Trube, G. Anion Dependence of the Contractions of skinned cardiac cells. Pflugers Arch. 379, 121–123 (1979). https://doi.org/10.1007/BF00586937
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DOI: https://doi.org/10.1007/BF00586937