Abstract
Adenosine produced by the macula densa cells in response to changes in the tubular NaCl-concentration has been suggested to inhibit renin release in vivo. In order to test this suggestion we studied: a) incubated kidney cortical slices (KS) which contain both the macula densa and the entire afferent arteriole; b) superfused single microdissected glomeruli (LAG) without macula densa but with the afferent artericole preserved; and c) superfused batches of selected glomeruli (SAG) containing only the juxtaglomerular cells closest to the glomerulus. For superfusion and incubation a bicarbonate Ringer solution was used. The specificity of the renin release process was validated by measuring adenylate kinase as a marker for cytoplasmatic leak.
Adenosine (10 μg/ml) halved basal renin release from incubated KS as compared to controls (P<0.001,n=8, 8). Renin release from LAG stimulated by calcium depletion was also inhibited (P<0.05,n=8, 9) whereas basal release was not affected (n=6, 12). No effect was detected neither on basal nor on calcium stimulated renin release from SAG.
We conclude that adenosine inhibits renin release in vitro by a mechanism independent of a functioning nephron, and which involves only the JG-cells located in the afferent arteriole at some distance from the glomerulus.
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Skøtt, O., Baumbach, L. Effects of adenosine on renin release from isolated rat glomeruli and kidney slices. Pflugers Arch. 404, 232–237 (1985). https://doi.org/10.1007/BF00581244
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DOI: https://doi.org/10.1007/BF00581244