Abstract
Marek's disease virus (MDV) is a highly cell-associated avian herpesvirus. In its natural host, MDV induces Marek's disease (MD), a lethal condition characterized by malignant lymphoma of T cells. Although symptoms of MD may be prevented by vaccination, no practical pharmacological method of control has been widely accepted. Viral replication represents a point at which pharmacological control of herpesvirus infection may be most successful. However, this requires detailed knowledge of viral replication proteins. Studies in HSV-1 DNA replication implicate the UL9 protein as a key initiator of replication. For example, binding of UL9 to HSV-1 origins is a prerequisite for assembly of additional replication proteins. In this study, a protein, whose apparent molecular size is similar to that of HSV-1 UL9, was identified in extracts of MDV infected cells by western blot analysis with anti-HSV-1 UL9 antibody. A putative MDV UL9 gene was subsequently identified through sequencing of MDV genome fragments (BamHI G and C). Extended DNA sequence analysis revealed an open reading frame (ORF) which could encode a protein homologous to HSV-1 UL9. The MDV UL9 ORF encodes 841 amino acids, producing a sequence 49% identical to HSV-1 UL9 and 46% identical to VZV gene 51 product (VZV UL9). MDV UL9 shares numerous structural motifs with HSV-1 and VZV UL9 proteins, including six conserved N-terminal helicase motifs, an N-terminal leucine zipper motif, a C-terminal pseudo-leucine zipper sequence, and a putative helix-turn-helix structure.
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CalnekB.W. and WitterR.L. in CalnekB.W., BarnesH.J., BeardC.W., ReidW.M., and YoderH.W.(Jr. eds.)Disease of Poultry. Iowa State University Press, Ames, Iowa, 1991, pp. 342–385.
RoizmanB. in FieldsB.N. and KnipeD.M. (eds.)Virology. Raven Press, New York, 1990, pp. 1787–1793.
BrunovskisP. and VelicerL.F. in Proceedings 19th World Poultry Congress Vol. 1, Ponsen & Looijen, Wageningen, The Netherlands, 1992, pp. 74–78.
BucmasterA.E., ScottS.D., SandersonM.J.S., BoursnellM.E.G., RossN.L.J., and BinnsM.M., J Gen Virol62, 2033–2042, 1988.
CebrianJ., Kaschka-DierichC., BerthelotN., and ScheldrickP., Proc Natl Acad Sci USA79, 555–558, 1982.
RoizmanB., Arch Virol123, 425–449, 1992.
CampH.S., CoussensP.M., and SilvaR. F., J Virol65, 6320–6324, 1991.
SmithG.D., ZelnikV., and RossL.N.J., Virology207, 205–216, 1995.
WuC.A., NelsonN.J., McGeochD.J., and ChallbergM.D., J Virol62, 435–443, 1988.
StowN.D. and McmonagleE.C., Virology130, 427–438, 1983.
WellerS.K., SpadaroA., SchafferJ.E., MurrayA.W., MaxamA.M., and SchafferP.A., Mol Cell Biol5, 930–942, 1985.
LockshonD. and GallowayD.A., Mol Cell Biol8, 4018–4027, 1988.
StowN.D., J Gen Virol66, 31, 1984.
EliasP. and LehmanI.R., Proc Natl Acad Sci USA85, 2959–2963, 1988.
MartinD.W., DebS.P., KlauerJ.S., and DebS., J Virol65, 4359–4369, 1991.
OlivoP.D., NelsonN.J., and ChallbergM.D. Proc Natl Acad Sci USA85, 5414–5418, 1988.
DebS. and DebS.P., Nucleic Acids Res17, 2733–2752, 1989.
WeirH.M. and StowN.D., J Gen Virol71, 1379–1385, 1990.
EliasP., GustaffsonC.M., and HammarstenO., J Biol Chem265, 17167–17173, 1990.
BrucknerR.C., CruteJ.J., DodsonM.S., and LehmanI.R., J Biol Chem266, 2669–2674, 1991.
BoehmerP.E., DodsonM.S., and LehmanI.R., J Biol Chem268, 1220–1225, 1993.
DodsonM.S. and LehmanI.R., J Biol Chem.268, 1213–1219, 1993.
MartinezR., ShaoL., and WellerS.K., J Virol66, 6735–6746, 1992.
DebS. and DebS.P., J Virol65, 2829–2838, 1991.
ChenD. and OlivoP.D., J Virol68, 3841–3949, 1994.
MartinD.W. and DebS., J Virol68, 3674–3681, 1994.
BaumanR.P., YalamanchiliV.R.R. and O'CallaghanD.J. J Virol63, 1275–1283, 1989.
StowN.D. and DavidsonA.J., J Gen Virol67, 1613–1623, 1986.
Camp H.S., Ph.D. Thesis, Department of Animal Science, Michigan State University, 1993.
EliasP., GustafssonC.M., HammarstenO., and StowN.D., J Biol Chem267, 17424–17429, 1992.
Hazuda D.J., Perry H.C., and McClements W.L., J Biol Chem 14309–14315, 1992.
ArbuckleM.I. and StowN.D., J Gen Virol74, 1349–1355, 1993.
GlaubigerC., NazarianK., and VelicerL.F., J Virol45, 1228–1234, 1983.
DabrowskiC.E. and SchafferP.A., J Virol65, 3140–3150, 1991.
ChomczynskiP. and SacchiN., Anal Biochem162, 156–159, 1987.
SambrookJ., FrisstschE.F., and MaiatisT., Molecular cloning: A Laboratory Manual 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1989.
KozakM., J Cell Biol108, 229–241, 1989.
McgeochD.M., DalrympleM.A., DolanA., McNabD., PerryL.J., TaylorP., and ChallbergM., J Virol62, 444–453, 1988.
RossL.J., SandersonM., ScottS.D., BinnsM.M., DoelT., and MilneB., J Gen Virol70, 1789–1805, 1989.
MartinD.W., MunozR.M., OliverD., SublerM.A., and DebS., Virology198, 71–80, 1994.
BaradaranK., DabrowskiC.E., and SchafferP.A. J Virol68, 4251–4261, 1994.
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Wu, TF., Sun, W., Boussaha, M. et al. Cloning and sequence analysis of a Marek's disease virus origin binding protein (OBP) reveals strict conservation of structural motifs among OBPs of divergent alphaherpesviruses. Virus Genes 13, 143–157 (1996). https://doi.org/10.1007/BF00568907
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DOI: https://doi.org/10.1007/BF00568907