Abstract
The isozyme pattern of superoxide dismutase (SOD) in tomato consists of two Cu,Zn isozymes located, respectively, in the chloroplast and in the cytosol, as well as additional isozymes of the Mn or Fe SOD type. We have shown that SOD-1 is the chloroplastic Cu,Zn SOD and is related to cDNA clone T10. Restriction fragment length polymorphism (RFLP) analysis was performed with two cDNA clones representing tomato Cu,Zn-superoxide dismutases. T10, coding for the chloroplast isozyme, was thus mapped to chromosome 11, between markerTG46 andTG108, while clone P31, coding for the cytosolic Cu,Zn SOD isozyme, was mapped to chromosome 1 betweenTG24 andTG81. SOD is associated with the response of plants to various environmental stresses; the mapping information presented here would permit the demonstration of this association by genetic analysis.
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This research was supported by a grant (No. 2730-1-86) funded by the European Community and the Israel National Council for Research and Development. Rafael Perl-Treves holds a Levi Eshkol scholarship funded by the Israel National Council for Research and Development.
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Perl-Treves, R., Abu-Abied, M., Magal, N. et al. Genetic mapping of tomato cDNA clones encoding the chloroplastic and the cytosolic isozymes of superoxide dismutase. Biochem Genet 28, 543–552 (1990). https://doi.org/10.1007/BF00554381
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DOI: https://doi.org/10.1007/BF00554381