Abstract
Two electrophoretic variants of the 6-phosphogluconate dehydrogenase (6PGD) enzyme have been found in the WHO/IN/Musca domestica/1 housefly laboratory strain. The patterns shown by Cellogel zone electrophoresis can be fully explained by the hypothesis of two codominant autosomal alleles. On this hypothesis, a specific Pgd locus has been postulated and the symbols Pgd A and Pgd B have been assigned to the two alleles causing the PGD-A and PGD-B phenotypes. The bands corresponding to the homozygous phenotypes PGD-A and PGD-B have different electrophoretic mobility and staining intensity; they can be described, respectively, as “fast-weak” and “slow-thick.” The heterozygous phenotype PGD-AB gives a three-banded pattern, indicative of a dimeric structure for this enzyme; this pattern is asymmetrical. Heterozygous flies have been found both among wild-type strains of recent colonization and among old established laboratory colonies. Most strains are Pgd B monomorphic; up to now only three strains have been Pgd A monomorphic, all of them being multimarker strains. The Pgd locus has been traced to the housefly linkage group III.
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This investigation was partially supported by Research Grants No. 76.01256.04 and No. 77.01419.04 from the Consiglio Nazionale delle Ricerche (C.N.R.), Rome, Italy.
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Gasperi, G., Malacrida, A. & Milani, R. 6-phosphogluconate dehydrogenase in the housefly, Musca domestica L.: Evidence for inheritable 6PGD polymorphism. Biochem Genet 17, 855–865 (1979). https://doi.org/10.1007/BF00504308
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DOI: https://doi.org/10.1007/BF00504308