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Neuron specific enolase (NSE) immunostaining a useful tool for the light microscopical detection of endocrine cell hyperplasia in adult rats exposed to asbestos

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Summary

Hyperplasia of endocrine cells in the lung of the adult rat exposed to asbestos has only been characterised so far by electron microscopy as there is a lack of reliable staining techniques for their demonstration at light microscopical level. Neuron specific enolase (NSE), an isoenzyme of the glycolytic enzyme enolase has recently been shown to be present in lung endocrine cells. In this study we reveal a marked endocrine cell hyperplasia at light microscopical level in the lungs of adult rats exposed to asbestos using antibodies to NSE. Very large groups of NSE-immunoreactive cells (20–80) were only observed in the lungs of rats exposed to asbestos for 12 months. In addition smaller groups of cells (2–10) known to be present normally and to decrease with age, were rarely noted in the controls but were frequently detected in the treated rats. Immunoreactive NSE is therefore a very good marker for endocrine cell hyperplasia and thus of early neoplastic changes.

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References

  • Becci PJ, McDowell EM, Trump BF (1978) The respiratory epithelium V1. Histogenesis of lung tumours induced by benzo(a)pyrene ferric oxide in the hamster. J Natl Cancer Inst 61:607–618

    Google Scholar 

  • Bensch KG, Gordon GB, Miller LR (1965) Electron microscopic and biochemical studies on the bronchial carcinoid tumour. Cancer 18:592–602

    Google Scholar 

  • Bensch KG, Corrin B, Panente R, Spencer H (1968) Oat cell carcinoma of the lung. Its origin and relationship to bronchial carcinoid. Cancer 22:1163–1172

    Google Scholar 

  • Bonikas DS, Archibald R, Bensch KG (1976) On the origin of the so-called tumourlets of the lung. Hum Pathol 7:461–469

    Google Scholar 

  • Churg A, Warnock ML (1976) Pulmonary tumourlets: A form of peripheral carcinoid. Cancer 37:1469–1477

    Google Scholar 

  • Cutz EC, Chan E, Wong V, Conen PE (1974) Endocrine cells in rat fetal lungs. Lab Invest. 30:458–464

    Google Scholar 

  • Edmondson NA, Lewis DJ (1980) Distribution and ultrastructural characteristics of Feyrter cells in the rat and hamster airway epithelium. Thorax 35:371–374

    Google Scholar 

  • Facer P, Polak JM, Marangos PJ et al. (1980) Immunocytochemical localization of neuron specific enolase (NSE) in the gastrointestinal tract. Proc R Microscop Soc 15:113–114

    Google Scholar 

  • Falck B, Owman Ch (1965) A detailed methodological description of the fluorescence method for cellular demonstration of biogenic monoamine. Acta Univ Lund Sectio II:71–123

    Google Scholar 

  • Gu J, Polak JM, Tapia FJ et al (1981) Neuron-specific enolase in the Merkel cells of mammalian skin. Am J Pathol 104:63–68

    Google Scholar 

  • Hage E (1972) Endocrine cells in the bronchial mucosa of human foetuses Acta Pathol Microbiol Scand 80:225–234

    Google Scholar 

  • Hage E (1973) Electron microscopic identification of several types of endocrine cells in the bronchial epithelium of human foetuses. Z Zellforsch 141:401–412

    Google Scholar 

  • Hage E, Hage J, Juel G (1977) Endocrine-like cells of the pulmonary epithelium of the human adult lung. Cell Tissue Res 178:39–48

    Google Scholar 

  • Jeffrey PK, Reid L (1975) New observations of rat airway epithelium: A quantitative and electron microscopical study. J Anat 120:295–320

    Google Scholar 

  • Johnson NF, Wagner JC, Wills HA (1980) Endocrine cell proliferation in the rat lung following asbestos inhalation. Lung 158:221–228

    Google Scholar 

  • Kleinerman J, Marchevsky AM, Thornton J (1981) Quantitative studies of APUD cells in airways of rats: The effects of diethylnitrosamine and NO2. Am Rev Respir Dis 124:458–462

    Google Scholar 

  • Lauweryns JM, Cokelaere M, Theunynack P (1972) Neuroepithelial bodies in the respiratory mucosa of various mammals. Z Zellforsch 135:569–592

    Google Scholar 

  • Linnoila RI, Nettesheim P, DiAugustine RP (1981) Lung endocrine-like cells in hamsters treated with diethylnitrosamine. Alterations in vivo and in cell culture. Proc Natl Acad Sci USA 78:5170–5174

    Google Scholar 

  • Marangos PJ, Schmechel D, Param A et al (1979) Measurement of neuron specific (NSE) and non-neuronal (NNE) isoenzymes of enolase in rat, monkey and human nervous tissue. J Neurochem 33:319–329

    Google Scholar 

  • McDowell EM, Sorokin SP, Hoyt RF, Trump BF (1979) Atypical neurosecretory cell tumours of the lung: An unrecognised entity. Lab Invest 40:272

    Google Scholar 

  • Moosavi H, Smith P, Heath D (1973) The Feyrter cell in hypoxia. Thorax 28:729–741

    Google Scholar 

  • Morson BC (1956) Intestinal metaplasia of the gastric mucosa. Gastroenterologia 85:181–190

    Google Scholar 

  • Morson BC, Pang L (1967) Rectal biopsy as an aid to cancer control in ulcerative colitis. Gut 8:423–434

    Google Scholar 

  • Palisano JR, Kleinerman J (1980) APUD cells and neuroepithelial bodies in hamster lung: methods, quantitation and response to injury Thorax 35:363–370

    Google Scholar 

  • Pearse AGE, Polak JM (1975) Bifunctional reagents as vapour- and liquid-phase fixatives for immunocytochemistry. Histochem J 7:179–186

    Google Scholar 

  • Pickel VM, Reis DJ, Marangos PJ et al (1976) Immunocytochemical localization of nervous system specific protein (NSP-R) in rat brain. Brain Res 105:184–187

    Google Scholar 

  • Reznik-Schuller H (1977) Ultrastructural alterations of APUD cells during nitrosamine induced lung carcinogenesis. J Pathol 121:79–82

    Google Scholar 

  • Schmechel DE, Marangos PJ, Brightman MW (1979) Neuron-specific enolase is a marker for peripheral and central neuroendocrine cells. Nature 276:834–836

    Google Scholar 

  • Solcia E, Vassallo G, Capella C (1968) Selective staining of endocrine cells by basic dyes after acid hydrolysis. Stain Technol 43:257

    Google Scholar 

  • Solcia E, Capella C, Vassallo G (1969) Lead haematoxylin as a stain for endocrine cells: significance of staining and comparison with other selective methods. Histochemie 20:116

    Google Scholar 

  • Sternberger LA (1979) Immunocytochemistry. 2nd ed. John Wiley and Sons, New York, pp 104–169

    Google Scholar 

  • Tateishi R (1973) Distribution of argyrophilic cells in adult human lungs. Arch Pathol 96:198–202

    Google Scholar 

  • Wagner JC, Berry G, Skidmore JW et al (1974) The effects of the inhalation of asbestos in rats. Br J Cancer 29:252–269

    Google Scholar 

  • Wharton J, Polak JM, Cole GA et al (1981) Neuron-specific enolase as an immunocytochemical marker for the diffuse neuroendocrine system in human foetal lung. J Histochem Cytochem 29:1359–1364

    Google Scholar 

  • Whitwell F, Newhouse ML, Bennett DR (1974) A study of the histological cell types of lung cancer in workers suffering from asbestosis in the United Kingdom. Br J Ind Med 31:298–303

    Google Scholar 

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Authors and Affiliations

  1. Departments of Histochemistry and Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London, UK

    M. N. Sheppard, N. F. Johnson, S. R. Bloom & J. M. Polak

  2. M.R.C. Pneumoconiosis Unit, Llandough Hospital, Penarth, Wales

    G. A. Cole

  3. Clinical Psychobiology Branch, National Institute of Mental Health, Maryland, USA

    P. J. Marangos

Authors
  1. M. N. Sheppard
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  2. N. F. Johnson
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  3. G. A. Cole
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  4. S. R. Bloom
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  5. P. J. Marangos
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  6. J. M. Polak
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Sheppard, M.N., Johnson, N.F., Cole, G.A. et al. Neuron specific enolase (NSE) immunostaining a useful tool for the light microscopical detection of endocrine cell hyperplasia in adult rats exposed to asbestos. Histochemistry 74, 505–513 (1982). https://doi.org/10.1007/BF00496664

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  • DOI: https://doi.org/10.1007/BF00496664

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