Summary
Histochemical investigation shows that ATPase activity is located intensively on the surface of cell contacts in hepatoma cells grown in confluent monolayer culture. Dibutyryl cyclic AMP and theophylline-treated hepatoma cells which exhibit contact-inhibited growth show the absence of localization of intense ATPase activity at cell-cell contacts. However, after removal of these additives the activity fully recovers to the intense level of control cells, when the release of cells from contact inhibition of growth occurs. Cultured hepatic parenchymal cells in monolayer have little or no ATPase activity at their surface immediately after contacts are established, and again after growth to a confluent state. In a different type of hepatoma cell which is less malignant and forms a small tissue mass or island, cell surface ATPase activity at cell-cell contacts in an island is very weak or scarcely detected even when cells are not treated with dibutyryl cyclic AMP and theophylline.
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References
Abercrombie, M., Ambrose, E.J.: The surface properties of cancer cells. A review. Cancer Res. 22, 525–548 (1962)
Garnett, H.M., Kemp, R.B.: (Ca2++Mg2+)-activated ATPase in plasma-membrane of mouse liver cells. Biochim. Biophys. Acta 382, 526–533 (1975)
Gwynn, I., Kemp, R.B., Jones, B.M.: Specific binding of anti-myosin and-actin γ-globulins to the surface of trypsin-dissociated embryonic chick cells. Cell Tissue Res. 171, 351–358 (1976)
Jones, B.M.: A unifing hypothesis of cell adhesion. Nature 212, 362–365 (1966)
Jones, P.C.T.: A contractile protein model for cell adhesion. Nature 212, 365–369 (1966)
Kanno, Y., Nomura, S., Matsui, Y.: The ruthenium red positive cell coat and electrical communication on the transplantable cancer cells (AH 7974). In Japanese. Symposium for Cell Biol. 24, 51–60 (1973)
Ohnishi, T., Kimura, S.: Contact-mediated changes in ATPase activity at the surface of primary cultured hepatoma cells. Histochemistry 49, 107–112 (1976)
Perissel, B., Charbonne, F., Chessebeuf, M., Malet, P.: Differentiation of the plasma membrane of hepatic cells in monolayer cultures. Cell Tissue Res. 171, 157–173 (1976)
Ronquist, G., Ågren, G.K.: A Mg2+-and Ca2+-stimulated adenosine triphosphatase at the outer surface of Ehrlich ascites tumor cells. Cancer Res. 35, 1402–1406 (1975)
Rosenthal, A.S., Kregenow, F.M., Moses, H.L.: Some characteristics of a Ca2+-dependent ATPase activity associated with a group of erythrocyte membrane proteins which form fibrils. Biochim. Biophys. Acta 196, 254–262 (1970)
Sheppard, J.R.: Restoration of contact-inhibited growth to transformed by dibutyryl adenosine 3′:5′-cyclic monophosphate. Proc. Nat. Acad. Sci. USA 68, 1316–1320 (1971)
Smets, L.A.: Contact inhibition of transformed cells incompletely restored by dibutyryl cyclic AMP. Nature [New Biol.] 239, 123–124 (1972)
Sugár, J.: Ultrastructural and histochemical changes during the development of cancer in various human organs. In: Tissue Interactions in Carcinogenesis, (ed. D. Tarin) p. 127–159. London and New York: Academic Press 1972
Takaoka, T., Yasumoto, S., Katsuta, H.: A simple method for the cultivation of rat liver cells. Japan. J. Exp. Med. 45, 317–326 (1975)
Teel, R.W., Hall, R.G.: Effect of dibutyryl cyclic AMP on the restoration of contact inhibition in tumor cells and its relationship to cell density and the cell cycle. Exp. Cell. Res. 76, 390–392 (1973)
Wernstedt, C.O., Ågren, G.K., Ronquist, G.: Enzyme activities at the surface of intact Ehrlich tumor cells with albumin in the isotonic assay medium. Cancer Res. 35, 1536–1541 (1975)
White, M.D., Ralston, G.B.: A water-soluble Mg2+-ATPase from erythrocyte membranes. Biochim. Biophys. Acta 436, 567–576 (1976)
Willingham, M.C., sOstlund, R.E., Pastan, I.: Myosin as a component of the cell surface of cultured cells. Proc. Nat. Acad. Sci. USA 71, 4144–4148 (1974)
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Yamaguchi, K., Ohnishi, T. Surface ATPase activity at cell-cell contacts in hepatic parenchymal cells and in cAMP-treated hepatoma cells in monolayer culture. Histochemistry 54, 191–199 (1977). https://doi.org/10.1007/BF00492241
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DOI: https://doi.org/10.1007/BF00492241