Summary
We examined rat cells undergoing amelogenesis for the presence of three types of keratin proteins using a polyclonal antibody to keratin (against total keratins (TK) with molecular masses ranging from 41 to 65 kilodaltons (kd) and monoclonal antibodies keratins to KL1 and PKK1 (reactive with keratins with molecular masses of 55–57 and 41–56 kd, respectively). In normal oral epithelia from young rats, the TK, KL1, and PKK1 antibodies bound to all of the epithelial strata. The epithelial cap on the top of incisors and the dental lamina of molar teeth exhibited strong TK staining, moderate staining KL1, and little or no PKK1 staining. In developing molar enamel organs, both the outer and inner enamel epithelia, the stratum intermedium, and stellate reticulum cells were all positively stained by the TK immunoreagent. In developing incisors, TK only bound strongly to stratum-intermedium cells, and no KL1 and PKK1 staining antibodies was observed in ameloblasts or the stratum intermedium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Banks-Schlegel SP, Harris CC (1982) Keratin alterations during embryonic epidermal differentiation: A presage of adult epidermal maturation. J Cell Biol 93:551–559
Banks-Schlegel SP, Schlegel R, Pinks GS (1981) Keratin protein domains within the human epidermis. Exp Cell Res 136:465–469
Franke WW, Appelhans B, Schmid E, Freudenstein C, Osborn M, Weber K (1979) Identification and characterization of epithelial cells in mammalian tissues by immunofluorescence microscopy using antibodies to prekeratin. Differentiation 15:7–25
Fuchs E, Green H (1980) Changes in keratin gene expression during terminal differentiation of the keratinocyte. Cell 19:1033–1042
Holthöfer H, Miettinen A, Lehto VP, Linder E, Alfthan O, Virtanen I (1983) Cellular origin and differentiation of renal carcinomas. A fluorescence microscopic study with kidney-specific antibodies, antiintermediate filament antibodies, and lectins. Lab Invest 49:317–326
Holthöfer H, Miettinen A, Lehto V-P, Lehtonen E, Virtanen I (1984) Expression of vimentin and in developing and adult human kidneys. Lab Invest 50:552–559
Kariniemi AL, Holthöfer H, Vartio T, Virtanen I (1984) Immunofluorescent localization of vimentin, prekeratin and actin during odontblast and ameloblast differentiation. Differentiation 21:133–137
Lesot H, Meyer JM, Rueh JV, Weber K, Osborn M (1985) Immunothuorescent localization of vimentin, prekeratin and actin during odontblast and ameloblast differentiation. Differentiation 21:133–137
Löning T, Staquet M-J, Schmitt D, Thivolet T (1982) Immunocytochemical and ultrastructural localization of keratin polypeptides in normal epidermal and mucosal cells and tissues. J Invest Dermatol 78:44–47
Moe H (1971) Morphological changes in the infranuclear portion of the enamel-producing cells during their life cycle. J Anat 108:43–62
Mori M, Ito M, Fukui S (1965) Decalcification for histochemical demonstration of hydrolytic and oxidative enzymes. Histochemistry 5:185–195
Nakai M, Tatemoto H, Mori H, Mori M (1985) Lectin-binding patterns in the developing tooth. Histochemistry 83:455–463
Said JW (1983) Immunohistochemical localization of keratin proteins in tumor diagnosis. Hum Pathol 14:1017–1019
Schlegel R, Banks-Schlegel S, Pinks GS (1980) Immunohistochemical localization of keratin in normal human tissues. Lab Invest 42:91–96
Sun TT, Eichner R, Nelson WG, Tseng SCG, Weiss RA, Jarvinen M, Woodcock-Mitchel J (1983) Keratin classes: Molecular markers for different types of epithelial differentiation. J Invest Dermatol 81:109–115
Sun TT, Eichner R, Schermer A, Cooper D, Nelson WG, Weiss RA (1984) Classification, expression, and possible mechanisms of evolution of mammalian epithelial keratins: A unifying model. In: Levine AJ et al. (eds) Cancer cells 1: The transformed phenotype. Cold Spring Harbor Laboratory, New York, pp 161–167
Takada K, Tamura K, Mori M (1968) Effect of Hg ion on histochemical demonstration of alkalin phosphatase in decalcified hard tissue. Acta Histochem Cytochem 1:37–42
Ten Cate AR (1962) The distribution of glycogen in human developing tooth. Arch Oral Biol 7:1–6
Ten Cate AR (1980) Oral histology development, structure, and function. CV Mosby, Saint Louis Toronto London
Thesleff I, Ekblom P (1984) Distribution of keratin and laminin in ameloblastoma. Comparison with developing tooth and epidermoid carcinoma. J Oral Pathol 13:85–96
Viac J, Reano A, Brochier J, Staquet N-J, Thivolet J (1983) Reactivity pattern of a monoclonal antikeratin antibody (KL1). J Invest Dermatol 81:351–354
Woodcock-Mitchell T, Eichner R, Nelson WG, Sun T-T (1983) Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol 95:580–588
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nakai, M., Tatemoto, Y., Mori, H. et al. Distribution profiles of keratin proteins during rat amelogenesis. Histochemistry 85, 89–94 (1986). https://doi.org/10.1007/BF00491753
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00491753