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Elemental composition of the dense bodies of rat platelets determined by electron probe X-ray microanalysis of freeze-substituted sections

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Summary

A semiquantitative x-ray microanalytical study was made of dense bodies in rat and human blood platelets prepared by freeze substitution. After the freeze substitution, electron dense bodies containing 5-hydroxytryptamine(5-HT) were well preserved. Electron probe x-ray microanalyses clearly defined species differences in the cations of dense bodies of rat and man. Direct calculation of elemental concentration ratios demonstrated a high level of Mg, with lesser amounts of K and Ca in rat, while there was a negligible level of Mg in man but a high amount of Ca. These results indicate that freeze-substitution is a suitable preparation for the study of diffusible electrolytes which may open the way to quantitative analysis using appropriate standards for analytical electron microscopy of resin sections.

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Authors and Affiliations

  1. Department of Anatomy School of Medicine, Teikyo University, Kaga, 2-11-1, Itabashi-ku, 173, Tokyo, Japan

    T. Daimon & K. Uchida

  2. Department of Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, Yushima, 1-5-45, Bunkyo-ku, 113, Tokyo, Japan

    V. Mizuhira & M. Ono

Authors
  1. T. Daimon
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  2. V. Mizuhira
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  3. M. Ono
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  4. K. Uchida
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Daimon, T., Mizuhira, V., Ono, M. et al. Elemental composition of the dense bodies of rat platelets determined by electron probe X-ray microanalysis of freeze-substituted sections. Histochemistry 77, 329–337 (1983). https://doi.org/10.1007/BF00490896

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  • DOI: https://doi.org/10.1007/BF00490896

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