Abstract
Polyacrylamide gel electrophoresis reveals similar differences between the retinal-specific lactate dehydrogenase (LDH) isozymes of the salmon (Salmo salar L.) and the sea-trout (Salmo trutta forma trutta L.) as those previously described for the salmon and the brown trout (Salmo trutta L.). F1 salmon × sea-trout hybrids give a classic hybrid isozyme pattern, but the F2 hybrids all possess the parental sea-trout type pattern. Loss of part of the salmon genome in these latter hybrids is the most likely explanation. It was observed that when the individual eye isozymes of the salmon, the sea-trout, and the rainbow trout (Salmo gairdneri Richardson) were eluted from preparative polyacrylamide gels and re-electrophoresed, an apparent interconversion of certain isozyme bands occurred. This phenomenon was also evident using starch gel. However, the major cathodally migrating isozyme in each case (presumably the E4 isozyme) re-electrophoresed pure. The reasons for these interconversions are, as yet, unclear. Attempts to produce in vitro hybridization between the various isolated individual isozymes were unsuccessful. Km pyruvate values for the different salmon isozymes were of the order expected from results already published for other teleosts.
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References
Alabaster, J. S., and Durbin, F. J. (1964). Blood groups in salmon, trout and their hybrids. Salmon Res. Trust Ireland Inc. Ann. Rep, Appendix IV.
Blanco, A., and Zinkham, W. H. (1963) Lactate dehydrogenase in human testes. Science 139601.
Dudman, N. P. B. (1969). Reconstitution of the electrophoretic complexity of mouse lactate dehydrogenase. Biochem. Biophys. Res. Commun. 36 608.
Dudman, N. P. B., and Zerner, B. (1969). Simplification of mouse lactate dehydrogenase electrophoretic patterns. Biochim. Biophys. Acta 171195.
Duke, E. J., and Kelly, M. (196). Lactate dehydrogenase isozymes in hybrids of salmon and sea-trout. Isozyme Bull. 1:20.
Epstein, C. J., and Schechter, A. N. (1968). An approach to the problem of conformational isozymes. Ann. N. Y. Acad. Sci. 15185.
Fantes, K. H., and Furminger, I. G. S. (1967). Protein, persulphate and disc electrophoresis. Nature 215750.
Fritz, P. J., and Jacobson, K. B. (1965). Multiple molecular forms of lactate dehydrogenase. Biochemistry 4282.
Goldberg, E. (1963). Lactic and malic dehydrogenase in human spermatozoa. Science 139602.
Goldberg, E. (1966) Lactate dehydrogenase of trout: Hybridization in vivo and in vitro. Science 1511091.
Goldberg, E., Cuerrier, J. P., and Ward, J. C. (1969). Lactate dehydrogenase ontogeny, paternal gene activation, and tetramer assembly in embryos of brook trout, lake trout and their hybrids. Biochem. Genet. 2335.
Haen, P. J., and O'Rourke, F. J. (1968). Proteins and haemoglobins of salmon-trout hybrids. Nature 21765.
Hitzeroth, H., Kloss, J., Ohno, S., and Wolf, U. (1968). Asynchronous activation of paternal alleles at the tissue-specific gene loci observed in hybrid trout during early development. Biochem. Genet. 1287.
Holmes, R. S., and Markert, C. L. (1969). Immunochemical homologies among subunits of trout lactate dehydrogenase isozymes. Proc. Natl. Acad. Sci. 64205.
Kitto, G. B., Wassarman, P. M., and Kaplan, N. O. (1966). Enzymatically active conformers of mitochondrial malate dehydrogenase. Proc. Natl. Acad. Sci. 56578.
Klose, J., Hitzeroth, H., Ritter, H., Schmidt, E., and Wolf, U. (1969). Persistence of maternal isoenzyme patterns of the lactate dehydrogenase and phosphoglucomutase system during early development of hybrid trout. Biochem. Genet. 391.
Lush, J. E., Cowey, C. B., and Knox, D. (1969). The lactate dehydrogenase isozymes of twelve species of flat-fish (Heterosomata). J. Exptl. Zool. 171105.
Markert, C. L. (1963). Lactate dehydrogenase isozymes: Dissociation and recombination of subunits. Science 1401329.
Markert, C. L. (1968). The molecular basis for isozymes. Ann. N. Y. Acad. Sci. 15114.
Markert, C. L., and Faulhaber, I. (1965). Lactate dehydrogenase patterns of fish. J. Exptl. Zool. 159319.
Markert, C. L., and Holmes, R. S. (1969). Lactate dehydrogenase isozymes of the flat-fish, Pleuronectiformes: Kinetic, molecular and immunochemical analysis. J. Exptl. Zool. 17185.
Markert, C. L., and Moller, F. (1959). Multiple forms of enzymes: Tissue, ontogenetic and species specific patterns. Proc. Natl. Acad. Sci. 45753.
Markert, C. L., and Whitt, G. S. (1968). Molecular varieties of isozymes. Experientia 24977.
Massaro, E. J., and Markert, C. L. (1968). Isozyme patterns of salmonid fishes: Evidence for multiple cistrons for lactate dehydrogenase polypeptides. J. Exptl. Zool. 168223.
Morrison, W. J., and Wright, J. E. (1966). Genetic analysis of three lactate dehydrogenase isozyme systems in trout: Evidence for linkage of genes coding for subunits A and B. J. Exptl. Zool. 163259.
Nachlas, M. M., Margulies, S. I., Goldberg, J. D., and Seligman, A. M. (1960). The determination of lactic dehydrogenase with a tetrazolium salt. Anal. Biochem. 1317.
Nyman, O. L. (1967). Protein variations in the Salmonidae. Inst. Freshwater Res. Drottningholm Rep. 475.
Nyman, O. L. (1970). Electrophoretic analysis of hybrids between salmon (Salmo salar, L.) and trout (Salmo trutta, L.). Trans. Am. Fish. Soc. 99229.
Ohno, S. (1969). The preferential activation of maternally derived alleles in development of interspecific hybrids. Wistaer Inst. Symp. Monogr. 9137.
Piggins, D. J. (1964). Salmon and sea-trout hybrids. Salmon Res. Trust Ireland Inc. Ann. Rep., Appendix III, p. 27.
Piggins, D. J. (1968). Salmon and sea-trout hybrids. Salmon Res. Trust Ireland Inc. Ann. Rep., Appendix XIII, p. 8.
Piggins, D. J. (1970). Salmon × sea-trout hybrids. Salmon Res. Trust Ireland Inc. Ann. Rep., Appendix I, p. 41.
Ressler, N. (1967). Interpretation of subbands of lactic dehydrogenase isozymes. Nature 215284.
Svardson, G. (1945). Chromosome studies on Salmonidae. Inst. Freshwater Res. Drottningholm Rep. 231.
Ursprung, H., and Carlin, L. (1968). Drosophila alcohol dehydrogenase: In vitro changes of isozyme patterns. Ann. N. Y. Acad. Sci. 1511156.
Whitt, G. S. (1970). Developmental genetics of the lactate dehydrogenase isozymes of fish. J. Exptl. Zool. 1751.
Whitt, G. S., Childers, W. F., and Wheat, T. E. (1971). The inheritance of tissue-specific lactate dehydrogenase isozymes in interspecific bass (Micropterus) hybrids. Biochem. Genet. 5257.
Wuntch, T., and Goldberg, E. (1970). A comparative physicochemical characterization of lactate dehydrogenase: Isozymes in brook trout, lake trout and their hybrid splake trout. J. Exptl. Zool. 174233.
Yen, T. T., and Glassman, E. (1965). Electrophoretic variants of xanthine dehydrogenase in Drosophila melanogaster. Genetics 52977.
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Joyce, P., Hearn, J., Kelly, M. et al. Genetic and biochemical aspects of lactate dehydrogenase isozymes in the salmonid eye. Biochem Genet 9, 327–342 (1973). https://doi.org/10.1007/BF00486068
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DOI: https://doi.org/10.1007/BF00486068