Abstract
The polypeptide compositions of fraction I protein isolated from six collections of Nicotiana glauca and from ten cultivars of N. tabacum, as well as a polyploid series and a male sterile line, have been analyzed by isoelectric focusing in polyacrylamide gel slabs containing 8 m urea. Apart from the male sterile line, none of the plants showed any variation from the species pattern of polypeptides. Fraction I protein from the male sterile Burley 21 cultivar of N. tabacum contained the two small subunit polypeptides of N. tabacum and the three large subunit polypeptides of an Australian species, probably N. megalosiphon. This indicates a changed chloroplast genome in the male sterile line in comparison to the normal fertile N. tabacum.
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Supported by Research Grant AI-00536 from the National Institutes of Health, U.S. Public Health Service, and by Contract AT(04-3)-34, Project 8, from the Division of Biology and Medicine, U.S. Atomic Energy Commission.
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Chen, K., Kung, S.D., Gray, J.C. et al. Polypeptide composition of fraction I protein from Nicotiana glauca and from cultivars of Nicotiana tabacum, including a male sterile line. Biochem Genet 13, 771–778 (1975). https://doi.org/10.1007/BF00484408
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DOI: https://doi.org/10.1007/BF00484408