Summary
Mitogenic activities for adult skin fibroblasts in 17 sera from untreated patients with scleroderma at the early stage were studied. Mitogenic activities in 10% test sera were measured by the [3H]-thymidine incorporation assay using confluent fibroblasts; there was no significnt difference between scleroderma-affected and normal controls. Growth-stimulatory activities for subconfluent fibroblasts in 10% test sera were measured by increased cell numbers; again, there was no significant difference between scleroderma-affected and normal controls.
There have been contradictory reports on growth-stimulatory activities in scleroderma sera; our data do not suggest the presence of a specific mitogen in scleroderma sera.
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Igarashi, A., Takehara, K., Soma, Y. et al. Mitogenic activities for skin fibroblasts in the sera from untreated scleroderma patients at the early stage. Arch Dermatol Res 281, 383–386 (1989). https://doi.org/10.1007/BF00455321
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DOI: https://doi.org/10.1007/BF00455321