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Fresh immature articular cartilage allografts

A Study on the Integration of Chondral and Osteochondral Grafts Both in Normal and in Papain-Treated Knee Joints of Rabbits

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Summary

The integration of immature cartilage allografts to knee joints of both normal rabbits and those submitted to arthritis through papain was studied for a period of up to 9 months.

Two types of grafts were compared: chondral—constituted only by cartilage, and osteochondral—cartilage plus a thin layer of subchondral bone.

The integration of the grafts was analyzed through inspection of the articular surface and histologic sections.

The viability of the grafts was checked through the incorporation of 35SO4 on the chondral matrix.

The following conclusions were drawn:

  1. (1)

    A large member of the grafted cartilages were well accepted without histological evidence of immunological rejection, and metabolically active 9 months after transplantation.

  2. (2)

    The chondral graft was found to be superior to the osteochondral one regarding the integration, but both showed tendency to degeneration with time.

  3. (3)

    The presence of arthritis previously induced by papain affected the integration of the grafts causing a higher percentage of degeneration on the grafted cartilage.

Zusammenfassung

Über einen Zeitraum bis zu 9 Monaten wurde die Integration unreifer Knorpel-Allotransplantate in den Kniegelenken normaler Ratten und solcher, bei denen mittels Papain Arthritis hervorgerufen wurde, beobachtet.

Es wurde ein Vergleich zwischen zwei Transplantatarten aufgestellt: 1. chondralen — bestehend aus nur einem Knorpel und 2. osteochondralen — bestehend aus einem Knorpel plus einer dünnen Schicht aus subchondralem Knochen.

Die Integration der Transplantate wurde analysiert durch Inspektion der Gelenkoberfläche und der histologischen Abschnitte.

Die Lebensdauer der Transplantate wurde durch Eingeben von 35SO4 in die Knorpelmatrize getestet.

Folgende Schlußfolgerungen wurden gezogen:

  1. 1.

    Ein Großteil der Knorpeltransplantate wurde gut aufgenommen, ohne histologischen Beweis für immunologische Abwehr, und war metabolisch noch nach 9 Monaten aktiv.

  2. 2.

    Das chondrale Transplantat integrierte sich besser als das osteochondrale, beide neigten jedoch mit der Zeit zu Degenerationserscheinungen.

  3. 3.

    Die zuvor mittels Papain hervorgerufene Arthritis beeinträchtigte die Integration der Transplantate und rief einen größeren Prozentsatz an degenerierten Knorpeltransplantaten hervor.

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References

  • Burwell, R. G.: Studies on the transplantation of bone. J. Bone Jt. Surg. 45-B, 386 (1963)

    Google Scholar 

  • Bacsich, P., Riddel, W. J. B.: Structure and nutrition of the cornea, cartilage and Wharton's jelly. Nature 155, 271 (1945)

    Google Scholar 

  • Bacsich, P., Wyburn, G. M.: The significance of the mucoprotein content on the survival of homografts of cartilage and cornea. Proc. Roy. Soc. (Edinburgh) 62, 321 (1947)

    Google Scholar 

  • Bentley, G.: Papain-induced degenerative arthritis of the hip joint in rabbits. J. Bone Jt. Surg. 53-B, 324 (1971)

    Google Scholar 

  • Bentley, G.: The effect of “biological” femoral osteotomy on papain-induced degenerative arthritis of the hip joint in rabbits. J. Bone Jt. Surg. 54-B, 173 (1972)

    Google Scholar 

  • Campbell, C. J.: Homotransplantation of a half or whole joint. Clin. Orthop. 87, 146 (1972)

    Google Scholar 

  • Craigmyle, M. G. L.: Studies of cartilage autografts and homografts in the rabbit. Brit. J. Plast. Surg. 8, 93 (1955)

    Google Scholar 

  • Campbell, C. J., Ishida, H., Takahashi, H., Kelly, F.: The transplantation of articular cartilage. An experimental study in dogs. J. Bone Jt. Surg. 45-A, 1579 (1963)

    Google Scholar 

  • De Palma, A. F., Tsaltas, T. T., Mauler, G. G.: Viability of osteochondral grafts as determined by uptake of 35S. J. Bone Jt. Surg. 45-A, 1565 (1963)

    Google Scholar 

  • Dupertuis, S. M.: Actual growth of young cartilage transplants in rabbits. Experimental studies. Arch. Surg. 43, 32 (1941)

    Google Scholar 

  • Gibson, T.: The transplantation of cartilage. J. Clin. Path. (Suppl.) 20, 513 (1967)

    Google Scholar 

  • Gibson, T., Davis, W. B., Curran, R. C.: The long-term survival of cartilage homografts in man. Brit. J. Plast. Surg. 11, 177 (1958)

    Google Scholar 

  • Gill, A. B.: The transplantation of entire bones with their joint surfaces. Ann. Surg. 61, 658 (1915)

    Google Scholar 

  • Herndon, C. H., Chase, S. W.: The fate of massive autogenous and homogenous bone grafts including articular surfaces. Surg. Gynecol. Obstet. 98, 273 (1954)

    Google Scholar 

  • Hesse, W., Hesse, I., Zech, G.: Regressive und reparative Vorgänge nach experimenteller Transplantation von homologem Gelenkknorpel. Arch. orthop. Unfall-Chir. 81, 89 (1975)

    Google Scholar 

  • Houpt, J. B., Langer, F., Pritzker, K. P. H., Gross, A.: Allotransplantation of joint surfaces in the treatment of osteonecrosis of the medial femoral condyle. Arthr. Rheum. 18, 405 (1975) (Abstract)

    Google Scholar 

  • Keiser, H., Dandson, J.: Immunology of cartilage proteoglycan. Fed. Proc. 32, 1474 (1973)

    Google Scholar 

  • Kettelkamp, D. B.: Experimental autologous joint transplantation. Clin. Orthop. 87, 138 (1972)

    Google Scholar 

  • Kopriwa, B. M., Leblond, C. P.: Improvements in the coating technique of radioautography. J. Histochem. Cytochem. 10, 269 (1962)

    Google Scholar 

  • Langer, F., Gross, A. F.: Immunogenicity of allograft articular cartilage. J. Bone Jt. Surg. 56-A, 297 (1974)

    Google Scholar 

  • Lloyd, G. J., McTavish, D. R., Soriano, S., Wiley, M. B., Young, M. H.: Fate of articular cartilage in joint transplantation. Can. J. Surg. 16, 306 (1973)

    Google Scholar 

  • Loeb, L.: Autotransplantation and homo-transplantation of cartilage in the guinea pig. Am. J. Path. 2, 111 (1926)

    Google Scholar 

  • McKibbin, B.: Immature joint cartilage and the homograft reaction. J. Bone Jt. Surg. 53-B, 123 (1971)

    Google Scholar 

  • McKibbin, B., Holdsworth, F. W.: The dual nature of epiphyseal cartilage. J. Bone Jt. Surg. 49-B, 351 (1967)

    Google Scholar 

  • Murray, D. G.: Experimentally induced arthritis using intraarticular papain. Arthr. Rheum. 7, 211 (1964)

    Google Scholar 

  • Paccola, C. A. J., Xavier, C. A. M.: Experimental arthritis through papain in rabbits. (In press)

  • Pap, K., Krompecher, S.: Arthroplasty of the knee. J. Bone Jt. Surg. 43-A, 523 (1961)

    Google Scholar 

  • Porter, B. B., Lance, E. M.: Limb and joint transplantation. Clin. Orthop. 104, 249 (1974)

    Google Scholar 

  • Pritzker, K. P. H., Gross, A. E., Langer, F., Luck, S. C., Houpt, J. B.: Articular cartilage transplantation. Human Path. 8, 635 (1977)

    Google Scholar 

  • Rothwell, A. G.: Cartilage repair in rabbit joints with papain induced arthritis. A preliminary report. Orthop. Oxford 6, 81 (1973)

    Google Scholar 

  • Seligman, G. M., George, E., Yablon, L: Transplantation of whole knee joints in the dog. Clin. Orthop. 87, 332 (1972)

    Google Scholar 

  • Sengupta, S.: The fate of transplants of articular cartilage in the rabbit. J. Bone Jt. Surg. 56-B, 167 (1974)

    Google Scholar 

  • Woodruff, M.: The transplantation of tissues and organs. Springfield/Ill.: Charles C. Thomas 1960

    Google Scholar 

  • Wyburn, G. M., Bacsich, P.: The uptake of labelled sulphate injected into the host animal by cartilage homograft. Brit. J. Plast. Surg. 8, 177 (1955)

    Google Scholar 

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Authors and Affiliations

  1. Department of Orthopaedic and Traumatologic Surgery School of Medicine of Ribeirao Preto, University of Sao Paulo, 14100, Ribeirao Preto, S.P., Brazil

    Cleber A. J. Paccola (Assistant Professor) & Camilo A. M. Xavier (Associate Professor)

  2. Department of Morphology School of Medicine of Ribeirao Preto, University of Sao Paulo, 14100, Ribeirao Preto, S.P., Brazil

    Renato P. Goncalves (Associate Professor)

Authors
  1. Cleber A. J. Paccola
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  2. Camilo A. M. Xavier
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  3. Renato P. Goncalves
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Paccola, C.A.J., Xavier, C.A.M. & Goncalves, R.P. Fresh immature articular cartilage allografts. Arch. Orth. Traum. Surg. 93, 253–259 (1979). https://doi.org/10.1007/BF00450223

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  • DOI: https://doi.org/10.1007/BF00450223

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