Abstract
Nitrate reductase of the salt tolerant alga Dunaliella parva, in contrast to that of most green algae, can use NADPH as well as NADH as electron donor. Extracts of cells contained various amounts of latent nitrate reductase. The latent enzyme could be activated at 45°C but only in the presence of flavine adenine dinucleotide. The heat activated enzyme did not require flavine adenine dinucleotide for activity and was fully active with NADH, NADPH or reduced flavine mononucleotide as electron donors.
Abbreviations
- FAD:
-
flavine adenine dinucleotide
- FMNH:
-
reduced flavine mononucleotide
References
Heimer, Y.M.: The effects of sodium chloride, potassium chloride and glycerol on the activity of nitrate reductase of a salt-tolerant and two non-tolerant plants. Planta (Berl.) 113, 279–281 (1973)
Losada, M., Herrera, J., Maldonado, J.M., Paneque, A.: Mechanism of nitrate reductase reversible inactivation by ammonia in Chlamydomonas. Plant Sci. Letters I, 31–37 (1973)
Maldonado, J.M., Herrera, J., Paneque, A., Losada, M.: Reversible inactivation by NADH and ADP of Chlorella fusca nitrate reductase. Bichem. biophys. Res. Commun. 51, 27–33 (1973)
McLachlan, J.: The culture of Dunaliella tertiolecta-Butcher, a euryhaline organism. Canad. J. Microbiol. 6, 367–379 (1960)
Rigano, C.: Studies on nitrate reductase from Cyanidium caldarium. Arch. Mikrobiol. 76, 265–276 (1971)
Rigano, C., Violante, U.: Effect of heat treatment on the activity in vitro of nitrate reductase from Cyanidium caldarium. Biochim. biophys. Acta (Amst.) 256, 524–532 (1972)
Solomonson, P. L.: Regulation of nitrate reductase activity by NADH and cyanide. Biochim. biophys. Acta (Amst.) 334, 297–308 (1974)
Wells, G.N., Hageman, R.H.: Specificity for nicotinamide adenine dinucleotide by nitrate reductase from leaves. Plant Physiol. 54, 136–141 (1974)
Wray, J.L., Filner, P.: Structural and functional relationships of enzyme activities induced by nitrate in barley. Biochem. J. 119, 715–725 (1970)
Zumft, W.G., Aparicio, P.J., Paneque, A., Losada, M.: Structural and functional role of FAD in the NADH-nitrate reducing system from Chlorella. FEBS Letters 9, 157–160 (1970)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Heimer, Y.M. Nitrate reductase of Dunaliella parva . Arch. Microbiol. 103, 181–183 (1975). https://doi.org/10.1007/BF00436347
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00436347