Summary
The MRS3 gene cloned in the multicopy plasmid YEp13 suppresses the mitochondrial splice defect exerted by mutation M1301 in the group II intron bIl. In this article we report on the behavior of the MRS3 gene cloned in the integration vector pEMBLYi27 and in the CEN4-ARS vector YCp50. Transformation of mutant M1301 cells with these recombinant vectors produced transformants, the majority of which showed the original splice defect and contained the recombinant vectors in single or low copy; a minority, however, was splicing competent and showed exceptionally high copy numbers of the MRS3 gene. These latter transformants had either the pEMBLYi27/MRS3 sequence repeated at least 20 times in tandem at the chromosomal site of the MRS3 gene or they had the YCp50/MRS3 sequence established as a multicopy plasmid lacking the copy number control usually exerted by the CEN4 sequence in this plasmid.
Similar content being viewed by others
References
Baldari C, Cesareni G (1985) Gene 35:27–32
Beggs JD (1978) Nature 275:104–109
Bitoun R, Zamir A (1986) Mol Gen Genet 204:98–102
Blackburn EH (1985) Trends Genet 1:8–12
Blackburn EH, Sostak JW (1984) Annu Rev Biochem 53:163–194
Broach JR, Strathern JN, Hicks J (1979) Gene 8:121–133
Clarke L, Carbon J (1980) Nature 287:504–509
Clarke L, Carbon J (1985) Annu Rev Gen 19:29–56
Conde J, Fink GR (1976) Proc Natl Acad Sci USA 69:930–932
Edlund T, Normak S (1981) Nature 292:269–271
Fishman SE, Rosteck PR, Hersberger JR, Hersberger CL (1985) J Bacteriol 161:199–206
Fogel S, Welch JW, Cathala G, Karin M (1983) Curr Genet 7:347–355
Hieter P, Mann C, Synder M, Davies RW (1985) Cell 40:381–392
Hinnen A, Hicks JB, Fink GR (1978) Proc Natl Acad Sci USA 75:1929–1933
Hyrien O, Buttin G (1986) Trends Genet 2:275–276
Kedes LH, Chang ACY, Houseman D, Cohen SN (1975) Nature 255:533–538
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor New York
Nasmyth K, Reed S (1980) Proc Natl Acad Sci USA 77:2119–2123
Orr-Weaver TL, Szostak JE, Rothstein RJ (1981) Proc Natl Acad Sci USA 78:6354–6385
Rigby PW, Dieckman M, Rhodes C, Berg P (1977) J Mol Biol 113:237–251
Schmelzer C, Schmidt C, May K, Schweyen RJ (1983) EMBO J 2:2047–2052
Schmidt C, Söllner T, Schweyen RJ (1987) Mol Gen Genet 210:145–152
Stark GR, Wahl GM (1984) Annu Rev Biochem 53:447–491
Southern EM (1975) J Mol. Biol 98:503–517
Stinchcomb DT, Mann C, Davis RW (1982) J Mol Biol 158: 157–179
Struhl K, Stinchcomb DT, Scherer S, Davis RW (1979) Proc Natl Acad Sci USA 76:1035–1039
Tlsty TD, Albertini AM, Miller JH (1984) Cell 37:217–224
Walton JD, Paquin CE, Koneko K, Williamson VM (1986) Cell 46:857–863
Winston F, Chumley F, Fink GR (1983) Recombinant DNA, part C. In: Wu R, Grossman L, Moldave K (eds) Methods. Enzymol 101:211–228
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Söllner, T., Schmidt, C. & Schmelzer, C. Amplification of the yeast nuclear gene MRS3 confers suppression of a mitochondrial RNA splice defect. Curr Genet 12, 497–501 (1987). https://doi.org/10.1007/BF00419558
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00419558