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Structural analysis of four strains of Paracoccus denitrificans

  • Development and Structure
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Abstract

Two out of eleven newly isolated strains of Paracoccus denitrificans were investigated by light and electron microscopic methods and compared with two strains of P. denitrificans already kept in culture collections. Samples were taken from different growth phases revealing short rods and nearly spherical cells in the exponential growth phase, and an increasing ratio of nearly spherical cells in the stationary growth phase. Cell division followed the binary fission mode; higher cell aggregates were not observed. Fine structural analysis revealed extracellular surface material stainable with Ruthenium red, a gram-negative cell wall and different storage material inclusions. Structural properties and variations within the four strains under investigation are discussed and compared with those of related bacteria.

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References

  • Aragno, M., Walther-Mauruschat, A., Mayer, F., and Schlegel, H. G. 1977. Micromorphology of gram-negative hydrogen bacteria. I. Cell Morphology and flagellation. — Arch. Microbiol. 114: 93–100.

    Google Scholar 

  • Behn, W. und Arnold, C. G. 1974. Die Wirkung von Streptomycin und Neamin auf die Chloroplasten- und Mitochondrien-Struktur von Chlamydomonas reinhardii. — Protoplasma (Wien) 82: 77–89.

    Google Scholar 

  • Beijerinck, M. W. und Minkman, D. C. J. 1910. Bildung und Verbrauch von Stickoxydul durch Bakterien. — Zbl. Bakt. II. Abt. 25: 53.

    Google Scholar 

  • Cheng, K.-J. and Costerton, J. W. 1977. Ultrastructure of Butyrivibrio fibrisolvens: a gram-positive bacterium. — J. Bacteriol. 129: 1506–1512.

    Google Scholar 

  • Cohen-Bazire, G. and Kunisawa, R. 1963. The fine structure of Rhodospirillum rubrum. — J. Cell Biol. 16: 401–419.

    Google Scholar 

  • Davis, D. H. 1967. Studies on the gram-negative hydrogen bacteria and related organisms. Thesis, University of California.

  • Davis, D. H., Doudoroff, M. Stanier, R. Y. and Mandel, M. 1969. Proposal to reject the genus Hydrogenomonas: taxonomic implications. — Int. J. Syst. Bact. 19: 375–390.

    Google Scholar 

  • Deutsche Sammlung von Mikroorganismen (DSM). 1977. Catalogue of strains, p. 70.

  • Doudoroff, M. 1974, p. 438–440. In R. E. Buchanan and N. E. Gibbons, (eds), Bergey's Manual of Determinative Bacteriology, 8th ed. — The Williams and Wilkins Co., Baltimore.

    Google Scholar 

  • Kluyver, A. J. 1956. Life's flexibility; microbial adaptation, p. 93–129. In A. J. Kluyver and C. B. van Niel, (eds), The microbes contribution to biology. — Harvard University Press, Cambridge, Massachusetts.

    Google Scholar 

  • Kocur, M., Martinec, T. and Mazanec, K. 1968. Fine structure of Micrococcus denitrificans and M. halodenitrificans in relation to their taxonomy. — Antonie van Leeuwenhoek 34: 19–26.

    Google Scholar 

  • Kran, G., Schlote, W. und Schlegel, H. G. 1963. Cytologische Untersuchungen an Chromatium okenii Petry. — Naturwissenschaften 50: 728–730.

    Google Scholar 

  • Luft, J. H. 1971. Ruthenium red and ruthenium violet. I. Chemistry, purification, methods of use for electron microscopy, and mechanism of action. — Anat. Res. 171: 347–368.

    Google Scholar 

  • Pfennig, N. 1974. Rhodopseudomonas globiformis, sp. n., a new species of the Rhodospirillaceae. —Arch. Microbiol. 100: 197–206.

    Google Scholar 

  • Schlegel, H. G., Kaltwasser, H. und Gottschalk, G. 1961. Ein Submersverfahren zur Kultur wasserstoffoxydierender Bakterien: Wachstumsphysiologische Untersuchungen. — Arch. Mikrobiol. 38: 209–222.

    Google Scholar 

  • Sleytr, U. and Kocur, M. 1973. Structure of Micrococcus denitrificans and M. halodenitrificans revealed by freeze-etching. — J. Appl. Bacteriol. 36: 19–22.

    Google Scholar 

  • Spurr, A. R. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. — J. Ultrastruct. Res. 26: 31–43.

    Google Scholar 

  • Valentine, R. C., Shapiro, B. M. and Stadtman, E. R. 1968. Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from E. coli. — Biochemistry 7: 2143–2152.

    Google Scholar 

  • Venable, J. H. and Coggeshall, R. 1965. A simplified lead citrate stain for use in electron microscopy. — J. Cell Biol. 25: 407–408.

    Google Scholar 

  • Verhoeven, W., Koster, A. L. and van Nievelt, M. C. A. 1954. Studies on true dissimilatory nitrate reduction. III. Micrococcus denitrificans Beijerinck, a bacterium capable of using molecular hydrogen in denitrification. — Antonie van Leeuwenhoek 20: 273–284.

    Google Scholar 

  • Vogt, M. 1965. Wachstumsphysiologische Untersuchungen an Micrococcus denitrificans Beij. —Arch. Mikrobiol. 50: 256–281.

    Google Scholar 

  • Walther-Mauruschat, A., Aragno, M., Mayer, F. and Schlegel, H. G. 1977. Micromorphology of gram-negative hydrogen bacteria. II. Cell envelope, membranes, and cytoplasmic inclusions. — Arch. Microbiol. 114: 101–110.

    Google Scholar 

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Nokhal, T.H., Mayer, F. Structural analysis of four strains of Paracoccus denitrificans . Antonie van Leeuwenhoek 45, 185–197 (1979). https://doi.org/10.1007/BF00418583

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