Summary
The aim of the study was to determine a biochemical basis for the augmented oxidative metabolism found in mononuclear leukocytes (MNL) of patients with active psoriasis. Dehydroepiandrosterone (DHEA) is known to inhibit glucose-6-phosphate dehydrogenase (G-6-PDH). We determined the activity of G-6-PDH as well as the penetration and metabolism of DHEA — diminished plasma concentrations of which have been found in psoriatics previously — in 16 patients with active psoriasis and 16 controls. MNL in patients with psoriasis possessed 52% more (p<0.05) G-6-PDH activity, based on cell number, and 34% more (p<0.05) activity, based on soluble protein. No difference in DHEA penetration and metabolism in MNL was found between psoriatics and controls, in contrast with previous findings of reduced penetration and increased reduction in erythrocytes of psoriatics. We conclude that the enhanced G-6-PDH activity in MNL of patients with active psoriasis is not due to altered DHEA penetration or metabolism.
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Altmeyer P, Munz DL, Chilf G, Holzmann H, Hör G (1983) Morphological and functional findings of fixed phagocytes in psoriasis. Arch Dermatol Res 275:95–99
Baxter JD, Funder JW (1979) Hormone receptors. N Engl J Med 301:1149–1161
Benes P, Simsony R, Oertel GW (1971) Inhibition of glucose-6-phosphate dehydrogenase by dehydroepian-drosterone and its conjugates. Steroidologia 2:52–56
Bergmeyer H-U (1970) Methoden der enzymatischen Analyse. Verlag Chemie, Weinheim, pp 599–607
Böyum A (1968) Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest [Suppl] 97:77–89
Brigelius R (1985) Mixed disulfides: Biological functions and increase in oxidative stress. In: Sies H (ed) Oxidative stress. Academic Press, London, pp 243–272
Czarnetzki B (1983) Increased monocyte chemotaxis towards leukotrine B 4 and platelet activating factor in patients with inflammatory dermatoses. Clin Exp Immunol 54:486–492
Geerdink JPM, de Muldner PHM, Franzen MR, Gommans JM, van Rennes H, van Erp PEJ, Bergers M, Mier PD (1984) Monocyte function is normal in quiescent psoriasis. J Invest Dermatol 82:122–125
Herlin T, Borregaard N, Kragballe K (1982) On the mechanisms of enhanced monocyte and neutrophil cytotoxicity in severe psoriasis. J Clin Invest 79:104–108
Higgs GA (1982) Arachidonic acid metabolism in leukocytes. In: Karnovsky ML, Bolis L (eds) Phagocytosis — past and future. Academic Press, New York, pp 105–129
Krueger GG, Jederberg WW, Ogden BE, Reese DL (1978) Inflammatory and immune cell function in psoriasis. J Invest Dermatol 71:195–201
Meuret G, Schmitt E, Hagedorn M (1976) Monocytopoiesis in chronic eczematous diseases, psoriasis vulgaris and mycosis fungoides. J Invest Dermatol 66:22–28
Morsches B, Benes P, Holzmann H (1979) Radioimmunologische Bestimmung von Dehydroepiandrosteron und 5-Androsten-3β,17β-diol im Plasma von Psoiratikern und Kontrollpersonen. Arch Dermatol Res 266:181–185
Morsches B, Benes P, Holzmann H, Henrich B (1971) Zur Penetrationskinetik von Dehydroepiandrosteron durch die Erythrozytenmembran. Arch Dermatol Res 270:49–55
Schopf RE, Altmeyer P, Lemmel E-M (1982) Increased respiratory burst activity of monocytes and polymorphonuclear leukocytes in psoriasis. Br J Dermatol 107:505–510
Schopf RE, Straussfeld E (1985) Stimulus-dependent increased generation of oxygen intermediates in monocytes and polymorphonuclear leukocytes in psoriasis. J Invest Dermatol 84:73–76
Traeger L (1977) Steroidhormon, Biosynthese, Stoffwechsel, Wirkung, 9. Aufl., Springer, Berlin Heidelberg New York
Tucker SB, Pierre RV, Jordon RE (1977) Rapid identification of monocytes in a mixed mononuclear cell preparation. I Immunol Methods 14:267–269
Voorhees JJ (1983) Leukotrienes and other lipoxygenase products in the pathogenesis and therapy of psoriatics and other dermatoses. Arch Dermatol 119:541–547
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In partial fulfillment of M.D. thesis, supported by a grant from the Deutscher Psoriasis-Bund
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Schopf, R.E., Müller, FJ., Benes, P. et al. Augmented glucose-6-phosphate dehydrogenase activity and normal penetration and metabolism of dehydroepiandrosterone in mononuclear leukocytes in psoriasis. Arch Dermatol Res 278, 393–397 (1986). https://doi.org/10.1007/BF00418169
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DOI: https://doi.org/10.1007/BF00418169