Abstract
The surface structures of the corneal epithelium of the heterozygous rhino mouse were examined by transmission electron microscopy and scanning electron microscopy. The surface structures of the corneal epithelium were composed of microvilli, microplicae and epithelial holes. Microvilli and microplicae showed the same features as those described previously. However, some microvilli bent at the top or stalk and looked like microplicae by scanning electron microscopy. The structure of epithelial holes was different from that reported previously. Epithelial holes with elevated rim demonstrated by scanning electron microscopy were formed by a large fold or process of epithelial cells in the outermost layer. Microvilli were present on the process (margin) and floor of epithelial holes. Thus microvilli on the floor belong to the outermost layer, not to the second layer.
Zusammenfassung
Die Oberflächenstruktur des Cornea-Epithels der Heterozygoten Rhino-Maus wurde transmissionselektronenmikroskopisch und rasterelektronenmikroskopisch untersucht. Die Oberflächenstruktur des Corneaepithels war zusammengesetzt aus Mikrovilli, Mikroplicae und epithelialen Hohlräumen. Die Mikrovilli und Mikroplicae zeigten die gleichen Eigenarten, die schon bisher beschrieben wurden. Einige Mikrovilli, welche an der Spitze oder am Stamm verbogen waren, sahen aus wie Mikroplicae im Rasterelektronenmikroskop. Die Struktur der epithelialen Löcher unterschied sich von den bisher beschriebenen. Epitheliale Hohlraumbildungen mit erhabenem Rand im Rasterelektronenmikroskop stellten sich dar als große Falten oder Fortsätze der epithelialen Zellen in der äußersten Schicht. Mikrovilli fanden sich sowohl am Rande wie auch auf dem Boden der epithelialen Hohlräume. Trotzdem gehören Mikrovilli auf dem Grunde zu der äußersten Zellschicht und nicht zu der zweiten Zellschicht.
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References
Blümcke, S., Morgenroth, K., Jr.: The stereo ultrastructure of the external and internal surface of the cornea. J. Ultrastruct. Res. 18, 502–518 (1967)
Caulfield, J.B.: Effects of varying the vehicle for OsO4 in tissue fixation. J. Biophys. Biochem. Cytol. 3, 827–830 (1957)
Hogan, M.J., Alvarado, J.A., Weddell, J.E.: Histology of the Human Eye. An Atlas and Textbook. p. 80. Philadelphia: Saunders 1971
Howard, A.: “Rhino,” an allele of hairless in the house mouse. J. Hered. 31, 467–470 (1940)
Kaye, G.I., Pappas, G.D.: Studies on the cornea. I. The fine structure of the rabbit cornea and the uptake and transport of colloidal particles by the cornea in vivo. J. Cell Biol. 12, 457–479 (1962)
Leuenberger, P.M.: Die Stereo-Ultrastruktur der Cornealoberfläche bei der Ratte. Albrecht v. Graefes Arch. Klin. Exp. Ophthalmol. 180, 182–192 (1970)
Pedler, C.: The fine structure of the corneal epithelium. Exp. Eye Res. 1, 286–289 (1962)
Pfister, R.R.: The normal surface of corneal epithelium: A scanning electron microscopic study. Invest. Ophthalmol. 12, 654–668 (1973)
Sheldon, H.: An electron microscope study of the epithelium in the normal mature and immature mouse cornea. J. Biophys. Biochem. Cytol. 2, 253–262 (1956)
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Amemiya, T., Yoshida, H., Yoshida, M. et al. Ultrastructures of the normal surface of corneal epithelium of the heterozygous rhino mouse with special reference to so-called epithelial holes. Albrecht von Graefes Arch. Klin. Ophthalmol. 213, 101–107 (1980). https://doi.org/10.1007/BF00413536
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DOI: https://doi.org/10.1007/BF00413536