Summary
Uptake of 14C-sorbose and 14C-3-O-methylglucose by ungerminated conidia of Neurospora crassa was measured by means of the millipore filter technique.
Initial rates of uptake of both sorbose and 3-O-methylglucose exemplify a marked dependence on pH of the incubation medium in the range between pH 3.5 and 6.5. The optimal pH for uptake of both sugars is close to 4.75.
When ungerminated conidia are “starved” with buffer for prolonged periods of time prior to assaying their transport capacity, in contrast to findings for germinated conidia and mycelia no de-repression of the glucose-repressible sugar transport system is effectuated.
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References
Beadle, G. W., Tatum, E. L.: Genetic control of biochemical reactions in Neurospora. Proc. nat. Acad. Sci. (Wash.) 27, 499–506 (1941).
Horowitz, N. H.: Methionine synthesis in Neurospora. The isolation of cystathionine. J. biol. Chem. 171, 255–264 (1947).
Klingmüller, W.: Aktive Aufnahme von Zuckern durch Zellen von Neurospora crassa unter Beteiligung eines enzymatischen Systems mit Permease-Eigenschaften, I. Z. Naturforsch. 22b, 181–188 (1967a).
—: Aktive Aufnahme von Zuckern durch Zellen von Neurospora crassa unter Beteiligung eines enzymatischen Systems mit permease-Eigenschaften, II. Z. Naturforsch. 22b, 188–195 (1967b).
—: Die Aufnahme der Zucker Sorbose, Fructose und Glucose durch Sorbose-resistente Mutanten von Neurospora crassa. Z. Naturforsch. 22b, 327–335 (1967c).
—: Kreuzungs-Analyse Sorbose-resistenter Mutanten von Neurospora crassa. Molec. Gen. Genetics 100, 109–116 (1967d).
—: Aufgliederung des Sorbose-Transportsystems von Neurospora crassa; Untersuchungen an Conidien und Protoplasten. Ber. dtsch. bot. Ges. 81, 303–305 (1968).
—: Cellular components specifically labelled during sorbose stimulation of sugar transport in Neurospora. Z. Naturforsch. 26b, 409–413 (1971).
Klingmüller, W. Huh, H.: Sugar transport in Neurospora. Submitted to Europ. J. Biochem.
Lester, G., Hechter, O.: Growth regulation in Penicillium puberulum by estradiol-17β and deoxycorticosterone. J. Bact. 81, 803–811 (1961).
Neville, M. M., Suskind, S. R., Roseman, S.: A derepressible active transport system for glucose in Neurospora crassa. J. biol. Chem. 246, 1294–1301 (1971).
Scarborough, G. A.: Sugar transport in Neurospora. J. biol. Chem. 245, 1694–1698 (1970a).
—: Sugar transport in Neurospora, II. A second glucose transport system. J. biol. Chem. 245, 3985–3987 (1970b).
Schneider, R. P., Wiley, W. R.: Kinetic characteristics of the two glucose transport systems in Neurospora crassa. J. Bact. 106, 479–486 (1971a).
——, Regulation of sugar transport in Neurospora crassa. J. Bact. 106, 487–492 (1971b).
——, Transcription and degradation of messenger ribonucleic acid for a glucose transport system in Neurospora. J. biol. Chem. 246, 4784–4789 (1971c).
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Klingmüller, W., Huh, H. Sugar transport in Neurospora conidia: Influence of pH and starvation. Archiv. Mikrobiol. 81, 239–244 (1972). https://doi.org/10.1007/BF00412242
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DOI: https://doi.org/10.1007/BF00412242