Summary
The nucleotide sequence of a 74 codon reading frame from the Aspergillus nidulans mitochondrial genome is presented. The derived amino acid sequence displays typical features of dicyclohexylcarbodiimide (DCCD) binding proteins and is 84% homologous with a mitochondrial reading frame that potentially encodes an ATPase subunit 9 polypeptide in Neurospora crassa. However, in A. nidulans, as in N. crassa, there is strong biochemical and genetic evidence that this subunit is in fact nuclearly-encoded. In both organisms the DCCD-binding protein found in the F0 complexes of mitochondria from actively-growing cultures is almost certainly the product of this nuclear gene, and definitely not that of the mitochondrial reading frame. The discovery of an intact open reading frame than can code for a DCCD-binding protein in the mitochondrial genome of a second species of filamentous fungus strenghthens the possibility that the presence of a mitochondrial version of this gene has some biological significance.
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References
Anderson S, Bankier AT, Barrell BG, de Bruijn MHL, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R, Young IG (1981) Nature 290:457–465
Anderson S, de Bruijn MHL, Coulson AR, Eperon IC, Sanger F, Young IG (1982) J Mol Biol 156:683–717
Bibb MJ, Van Etten RA, Wright CT, Walberg WM, Clayton DA (1981) Cell 26:167–180
van den Boogaart P, Samallo J, Agsteribbe E (1982) Nature 298:187–189
Brown TA, Davies RW, Waring RB, Scazzocchio C (1983) Nature 302:721–723
Grisi E, Brown TA, Waring RB, Scazzocchio C, Davies RW (1982) Nucleic Acids Res 10:3531–3539
Lazarus CM, Lunsdorf H, Hahn U, Stepien PP, Kuntzel H (1980) Mol Gen Genet 177:389–397
Orian JM, Murphy M, Marzuki S (1981) Biochem Biophys Acta 652:234–239
Rowlands RT, Turner G (1975) Mol Gen Genet 141:69–79
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Sebald W, Hoppe J, Wachter E (1979) In: Quagliariello E (ed) Function and molecular aspects of biomembrane transport. Elsevier, North Holland, Amsterdam, pp 63–74
Sebald W, Machleidt M, Wachter E (1980) Proc Natl Acad Sci USA 77:785–789
Turner G, Imam G, Kuntzel H (1979) Eur J Biochem 97:565–571
Viebrock A, Perz A, Sebald W (1982) EMBO J 1:565–571
Waring RB, Davies RW, Lee S, Grisi E, Berks MM, Scazzocchio C (1981) Cell 27:4–11
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Brown, T.A., Ray, J.A., Waring, R.B. et al. A mitochondrial reading frame which may code for a second form of ATPase subunit 9 in Aspergillus nidulans . Curr Genet 8, 489–492 (1984). https://doi.org/10.1007/BF00410434
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DOI: https://doi.org/10.1007/BF00410434