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Identification of new peptides synthesized under the hydrogenase control system of Alcaligenes eutrophus

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Abstract

Total protein of Alcaligenes eutrophus was analyzed by two-dimensional protein map. Cells grown at 30° C expressed hydrogen-oxidizing (Hox) ability mediated by a soluble (Hos) and a particulate hydrogenase (Hop). Hox ability was not expressed at 37° C (HoxTs). The six subunits of the two hydrogenases were identified. Besides these six subunits eight peptides were not or hardly detected at 37° C. The mutant HF117 which expressed Hox ability at 37° C (HoxTr), formed the hydrogenase peptides and five of the eight peptides. These peptides designated B, C, E, F, and H were characterized by their isoelectric point and molecular mass (M r); their M r were 18 800, 45 400, 41 900, 39 400, and 40 600, respectively. The five peptides were not formed in regulatory Hox− mutants, and not formed in mutants cured of plasmid pHG1, carrying the genetic information for hydrogenase formation. Strain HF160, carrying a Tn5 insertion in a gene essential for Hos expression specifically did not form the B-peptide. All peptides were found in the soluble fraction of cell extracts, the F-peptide was also detected in the particulate fraction. The function of the new Hox-peptides is presently unknown.

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Abbreviations

PAGE:

polyacrylamide gelelectrophoresis

SDS:

sodium dodecylsulfate

Hox:

hydrogen oxidizing ability

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Author notes

  1. U. Kärst

    Present address: Gesellschaft für Biotechnologische Forschung mbH, D-3300, Braunschweig-Stöckheim, FRG

Authors and Affiliations

  1. Institut für Mikrobiologie der Universität Göttingen, Grisebachstraße 8, D-3400, Göttingen, Federal Republic of Germany

    U. Kärst & C. G. Friedrich

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  1. U. Kärst
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  2. C. G. Friedrich
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Kärst, U., Friedrich, C.G. Identification of new peptides synthesized under the hydrogenase control system of Alcaligenes eutrophus . Arch. Microbiol. 147, 346–353 (1987). https://doi.org/10.1007/BF00406131

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