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Human allospecific TLCs generated against HLA antigens associated with DRl through DRw8

III. Family segregation analyses

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Abstract

We have studied the complexity and fine specificity of the HLA-D region using a panel of T lymphocyte clones generated against alloantigens associated with HLA-DR1 through DRw8. After extensive testing in population studies, 89 clones were tested in proliferation assays with 14 families. Segregation patterns were analyzed for haplotype associations by calculating sequential lod scores to test the likelihood that genes encoding epitopes detected by TLCs were linked to HLA genes. Four general categories were identified: (1) clonal responses that segregated with the same HLA-D region haplotype in all informative pedigrees; (2) clonal responses that segregated with HLA in all pedigrees but not always with the same haplotype; (3) clonal responses that segregated with HLA in some families but failed to segregate in others or produced equivocal results; (4) clonal responses that did not segregate with HLA haplotypes.

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Abbreviations

cpm:

counts per minute

DNV:

double normalized value

EBV:

Epstein-Barr virus

FCS:

fetal calf serum

HLA :

human MHC

HTC:

homozygous typing cell

LCL:

lymphoblastoid cell line

MHC:

major histocompatibility complex

MLC:

mixed lymphocyte culture

mAb:

monoclonal antibody

PBL:

peripheral blood lymphocyte

PLT:

primed lymphocyte typing

T-max:

maximized T test analysis

TCGF:

T-cell growth factor

TLC:

T-lymphocyte clone

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Authors and Affiliations

  1. Laboratory of Immunogenetics, NIAID, NIH, Bethesda, Maryland, USA

    Sandra Rosen-Bronson

  2. Lombardi Cancer Center, Georgetown University School of Medicine, Washington, D. C., USA

    Armead H. Johnson

  3. Transplantation and Immunology Branch, Naval Medical Research Institute, Bethesda, Maryland, USA

    Robert J. Hartzman

  4. Immunogenetics Research Section, The Blood Center of Southeastern Wisconsin, Milwaukee, Wisconsin, USA

    David D. Eckels

Authors
  1. Sandra Rosen-Bronson
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  2. Armead H. Johnson
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  3. Robert J. Hartzman
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  4. David D. Eckels
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Rosen-Bronson, S., Johnson, A.H., Hartzman, R.J. et al. Human allospecific TLCs generated against HLA antigens associated with DRl through DRw8. Immunogenetics 27, 31–39 (1988). https://doi.org/10.1007/BF00404441

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  • DOI: https://doi.org/10.1007/BF00404441

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