Summary
When studying mutants affecting lysyl-tRNA synthetase or tRNALys (hisT, hisW), a lack of correlation is clearly observed between the amount of lysyl-tRNA and the level of derepression of several lysine biosynthetic enzymes. This excludes the possible role of lysyl-tRNA as the specific corepressor of the lysine regulon. However, the level of derepression of DAP-decarboxylase, the last enzyme of the lysine pathway, is very low in the hisT mutant; this indicates that tRNALys is a secondary effector involved in the regulation of the synthesis of this enzyme.
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Abbreviations
- DAP:
-
diaminopimelate
- KRS:
-
lysyl-tRNA synthetase
- L-lysine:
-
tRNA ligase (AMP) (EC6.1.16)
- AK III:
-
lysinesensitive aspartokinase (EC 2.7.24)
- ASA-dehydrogenase:
-
aspartic semialdehyde dehydrogenase (EC 1.2.1.10)
- DHDP-reductase:
-
dihydrodipicolinic acid reductase
- DAP-decarboxylase:
-
diaminopimelate decarboxylase (EC 4.1.1.20)
- AK I:
-
threonine-sensitive aspartokinase
- HDHI:
-
threonine-sensitive homoserine dehydrogenase
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Communicated by F. Gros
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Boy, E., Borne, F. & Patte, JC. Effect of mutations affecting lysyl-tRNALys on the regulation of lysine biosynthesis in Escherichia coli . Molec. Gen. Genet. 159, 33–38 (1978). https://doi.org/10.1007/BF00401745
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DOI: https://doi.org/10.1007/BF00401745