Abstract
Changes in gas-exchange rates during the life span of the leaves of rice (Oryza sativa L.) were analyzed quantitatively by measuring changes in the carboxylation/oxygenation and regeneration of ribulose 1,5-bisphosphate (RuBP) at photon fluence rates of 2000 (saturating) and 500 (subsaturating) μmol quanta·m-2·s-1 under ambient air conditions. The RuBP levels were always higher than the active-site concentrations of RuBP carboxylase (EC 4.1.1.39), irrespective of the irradiance supplied. Analysis of the CO2-assimilation rate as a function of intercellular CO2 concentration indicated that RuBP regeneration does not limit CO2 assimilation. The estimated RuBP-carboxylase/oxygenase activity in vivo was linearly correlated to the rate of CO2 assimilation at each level of irradiance. This enzyme activity was just enough to account for the rate of CO2 assimilation at the saturating irradiance and was 35% more than the rate of CO2 assimilation at the subsaturating irradiance. Analysis of the assimilation rate at subsaturating irradiance as a function of intercellular CO2 concentration indicated that a limitation caused by enzyme activation comes into play. The results indicate that the rate of CO2 assimilation in rice leaves under ambient air conditions is limited during their entire life span by the RuBP-carboxylation/oxygenation capacity.
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Abbreviations
- DTT:
-
dithiothreitol
- RuBP:
-
ribulose 1,5-bisphosphate
References
Arnon, D.I. (1949) Copper enzyme is isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol. 24, 1–15
Badger, M.R., Sharkey, T.D., von Caemmerer, S. (1984) The relationship between steady-state gas exchange of bean leaves and the levels of carbon-reduction-cycle intermediates. Planta 160, 305–313
Bahr, J.T., Jensen, R.G. (1978) Activation of ribulose bisphosphate carboxylase in intact chloroplasts by CO2 and light. Arch. Biochem. Biophys. 185, 39–48
Chonan, N. (1967) Studies on the photosynthetic tissues in the leaves of cereal crops. III. The mesophyll structure of rice leaves inserted at different levels of the shoot. Jpn. J. Crop Sci. 36, 291–296
Chonan, N. (1970) Studies on the photosynthetic tissues in the leaves of cereal crops. V. Comparison of the mesophyll structure among seedlings leaves of cereal crops. Jpn. J. Crop Sci. 39, 418–425
Evans, J.R. (1983) Nitrogen and photosynthesis in the flag leaf of wheat (Triticum aestivum L.) Plant Physiol 72, 297–302
Evans, J.R., Seemann, J.R. (1984) Differences between wheat genotypes in specific activity of ribulose-1,5-bisphosphate carboxylase and the relationship to photosynthesis. Plant Physiol. 74, 759–765
Farquhar, G.D. (1979) Models describing the kinetics of ribulose bisphosphate carboxylase-oxygenase. Arch. Biochem. Biophys. 193, 456–468
Farquhar, G.D., von Caemmerer, S. (1982) Modelling of photosynthetic response to environmental conditions. In: Encyclopedia of plant physiology, N.S., vol. 12B: Physiological plant ecology II: Water relations and carbon assimilation, pp. 549–587, Lange, O.L., Nobel, P.S., Osmond, C.B., Ziegler, H., eds. Springer, Berlin Heidelberg New York
Kariya, K., Tsunoda, S. (1980) Size and shape of the chloroplasts existing in and isolated from mesophyll cell and their surface proportion contacted with cell wall in several crops. Jpn. J. Crop Sci. 49, 602–607
Mae, T., Ohira, K. (1981) The remobilization of nitrogen related to leaf growth and senescence in rice plants (Oryza sativa L.). Plant Cell Physiol. 22, 1067–1074
Mae, T., Ohira, K. (1983) Origin of the nitrogen of a growing rice leaf and its relation to nitrogen nutrition. Jpn. J. Soil. Sci. Plant Nutr. 54, 401–405
Makino, A., Mae, T., Ohira, K. (1983a) Purification and storage of ribulose-1,5-bisphosphate carboxylase from rice leaves. Plant Cell Physiol. 24, 1169–1173
Makino, A., Mae, T., Ohira, K. (1983b) Photosynthesis and ribulose-1,5-bisphosphate carboxylase in rice leaves. Changes in photosynthesis and enzymes involved in carbon assimilation from leaf development through senescence. Plant Physiol. 73, 1002–1007
Makino, A., Mae, T., Ohira, K. (1984a) Relation between nitrogen and ribulose-1,5-bisphosphate carboxylase in rice leaves from emergence through senescence. Plant Cell Physiol. 25, 429–437
Makino, A., Mae, T., Ohira, K. (1984b) Changes in photosynthetic capacity in rice leaves from emergence through senescence. Analysis from ribulose-1,5-bisphosphate carboxylase and leaf conductance. Plant Cell Physiol. 25, 511–521
Makino, A., Mae, T., Ohira, K. (1985) Enzymic properties of ribulose-1,5-bisphosphate carboxylase/oxygenase purified from rice leaves. Plant Physiol. 79, in press
Murata, Y., Matsushima, S. (1975) Rice. In: Crop physiology, pp. 73–99, Evans, L.T., ed. Cambridge University Press, London
Parker, M.C., Ford, M.A. (1982) The structure of the mesophyll of flag leaves in three Triticum species. Ann. Bot. 49, 165–176
Perchorowicz, J.T., Raynes, D.A., Jensen, R.G. (1981) Light limitation of photosynthesis and activation of ribulose bisphosphate carboxylase in wheat seedlings. Proc. Natl. Acad. Sci. USA 78, 2985–2989
Perchorowicz, J.T., Jensen, R.G. (1983) Photosynthesis and activation of ribulose bisphosphate carboxylase in wheat seedlings. Regulation by CO2 and O2. Plant Physiol. 71, 955–960
Raven, J.A., Gildewell, S.M. (1981) Processes limiting carboxylation efficiency. In: Processes limiting plant productivity, pp. 109–136, Johnson, C.B., ed. Butterworths, London
Sasahara, T. (1982) Influence of genome on leaf anatomy of Triticum and Aegilops. Ann. Bot. 50, 491–497
Takeda, T., Murata, H. (1956) Studies on CO2 exchange in crop plants. IV. Roles played by the various parts of the photosynthetic organs of rice plant in producing grains during ripening period. Jpn. J. Crop Sci. 24, 181–184
Tanaka, A. (1958) Studies on the characteristics of the physiological function of leaf at definite position on stem of rice plant. XI. Comparison of photosynthetic activity of leaves at various positions of main stem and translocation of photosynthetic products within plant. Jpn. J. Soil. Sci. Plant Nutr. 29, 327–332
Thorne, G.N. (1965) Physiological aspects of grain yield in cereals. In: The growth of cereals and grasses, pp. 88–105, Milthorpe, F.L., Ivins, J.D., eds. Butterworths, London
Tsunoda, S., Khan, A.H. (1968) Differences among strains of rice in the photosynthetic tissues. I. A comparative leaf anatomy of Indica and Japonica. Tohoku J. Agric. Res. 19, 1–7
von Caemmerer, S., Farquhar, G.D. (1981) Some relationships between the biochemistrry of photosynthesis and the gas exchange of leaves. Planta 153, 376–387
von Caemmerer, S., Farquhar, G.D. (1984) Effects of partial defoliation, changes of irradiance during growth, short-term water stress and growth at enhanced p(CO2) on the photosynthetic capacity of leaves of Phaseolus vulgaris L. Planta 160, 320–329
Yoshida, S. (1981) Rice plant characters in relation to yielding ability. In: Fundamentals of rice crop science, pp. 213–230, Yoshida, S., ed. International Rice Research Institute, Los Baños, Philippines
Yoshida, S., Cock, J.H. (1971) Growth performance of an improved rice variety in the tropics. Int. Rice Commun. Newslett. 20, 1–15
Zurawski, G., Perrot, B., Bottomley, W., Whitfeld, P.T. (1981) The structure of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase from spinach chloroplast DNA. Nucleic Acids Res. 9, 3251–3270
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Makino, A., Mae, T. & Ohira, K. Photosynthesis and ribulose-1,5-bisphosphate carboxylase/oxygenase in rice leaves from emergence through senescence. Quantitative analysis by carboxylation/oxygenation and regeneration of ribulose 1,5-bisphosphate. Planta 166, 414–420 (1985). https://doi.org/10.1007/BF00401181
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DOI: https://doi.org/10.1007/BF00401181