Summary
A double mutant being thermoconditionally defective in mutation induction as well as in repair of pre-lethal UV-induced DNA damage (rev2 ts) and deficient in excision repair (rad3-2) was studied in temperature-shift experiments. The influence of inhibitors of DNA replication (hydroxyurea, aphidicolin) was determined. Additionally, an analysis of the dose-response pattern of mutation induction (“mutation kinetics”) at several ochre alleles was carried out. It was concluded that the UV-inducible REV2 dependent mutagenic repair process is not induced in excision-deficient cells. In excision-deficient cells, REV2 dependent mutation fixation is slow and mostly postreplicative though not dependent on DNA replication. The REV2 mediated mutagenic process could be separated from the repair function.
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Siede, W., Eckardt, F. Analysis of mutagenic DNA repair in a thermoconditional mutant of Saccharomyces cerevisiae . Curr Genet 10, 871–878 (1986). https://doi.org/10.1007/BF00398283
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DOI: https://doi.org/10.1007/BF00398283