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Analysis of coenzyme Q systems, monosaccharide patterns of purified cell walls, and RAPD-PCR patterns in the genus Kluyveromyces

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Abstract

Analysis of the coenzyme Q system and the monosaccharide pattern of purified cell walls were used for species characterization in the genus Kluyveromyces. All the type strains of the genus possess coenzyme Q-6 and the mannose-glucose (‘Saccharomyces type’) cell wall sugar pattern. With the help of Random Amplified Polymorphic DNA-Polymerase Chain Reaction analysis 17 species were separated: K. aestuarii, K. africanus, K. bacillisporus, K. blattae, K. delphensis, K. dobzhanski, K. lactis (anamorph Candida sphaerica), K. lodderae, K. marxianus (syn. K. fragilis, K. bulgaricus, K. cicerisporus anamorphs Candida macedoniensis, C. pseudotropicalis, C. kefyr), K. phaffii, K. piceae, K. polysporus, K. sinensis, K. thermotolerans (syn. K. veronae, anamorph Candida dattila), K. waltii, K. wickerhamii, K. yarrowii (anamorph Candida tannotolerans). A strain of K. drosophilarum showed with the type strain of K. lactis only 63% similarity. The strain originally described as the type strain of K. cellobiovorus nom. nud. was excluded from the genus (Q-9), and found to be conspecific with the type strain of Candida intermedia.

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Abbreviations

nDNA:

nuclear DNA

RAPD-PCR:

Random Amplified Polymorphic DNA-Polymerase Chain Reaction

T :

type strain of the epithet

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Authors and Affiliations

  1. Inst. f. Angew. Mikrobiologie, Universität für Bodenkultur, Nußdorfer Lände 11, A-1190, Wien, Austria

    Orsolya Molnár, Hansjörg Prillinger & Ksenija Lopandic

  2. Hewlett-Packard GmbH, Lieblgasse 1, A-1222, Wien, Austria

    Franz Weigang

  3. Inst. f. Chemie, Universität für Bodenkultur, Gregor-Mendel-Straße 33, A-1180, Wien, Austria

    Erika Staudacher

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  1. Orsolya Molnár
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  2. Hansjörg Prillinger
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  3. Ksenija Lopandic
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  4. Franz Weigang
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  5. Erika Staudacher
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Molnár, O., Prillinger, H., Lopandic, K. et al. Analysis of coenzyme Q systems, monosaccharide patterns of purified cell walls, and RAPD-PCR patterns in the genus Kluyveromyces . Antonie van Leeuwenhoek 70, 67–78 (1996). https://doi.org/10.1007/BF00393571

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