Summary
Uridine diphosphoglucose is not the sole donor for trehalose synthesis in yest cells: an ADPG-dependent trehalose synthase, has been identified in mutant strains with undetectable UDPG-dependent trehalose-6-P synthase activity. Genetic and chromatographic studies indicate that the two activities correspond to different proteins. The apparent K Km for the nucleotide is similar for both enzymes, and Mg2+ is also required for both activities; however, a striking difference was observed with respect to ATP.Mg activation. This newly determined enzymatic activity in Saccharomyces clarifies previous contradictory results with mutant strains that are able to accumulate trehalose during growth yet whose UDPG-dependent trehalose synthase activity is undetectable in vitro.
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Abbreviations
- PMSF :
-
Phenyl-methyl-sulfonyl fluoride
- EDTA :
-
ethileno-daminotetracetic acid
- G6P :
-
glucose-6-phosphate
- UDPG :
-
uridine5′-diphosphoglucose
- ADPG :
-
adenosine-5′-diphosphoglucose
- UDP :
-
uridine-5′-diphosphate
- ADP :
-
adenosine-5′-diphosphate
- PEP:
-
phosphoenol pyruvate
- ATP :
-
adenosine-5′-triphosphate
- UTP :
-
uridine-5′triphosphate
- Pi :
-
inorganic phosphate
- MOPS 3:
-
(N-morpholino) propanesulfonic acid
- PNPG :
-
paranitrophenylglucoside
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Paschoalin, V.M.F., Silva, J.T. & Panek, A.D. Identification of an ADPG-dependent trehalose synthase in Saccharomyces . Curr Genet 16, 81–87 (1989). https://doi.org/10.1007/BF00393399
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DOI: https://doi.org/10.1007/BF00393399