Abstract
A calcium-binding macromolecule, with an estimated molecular weight greater than 100,000, was detected in phloem exudate from Cucurbita maxima and related species. The macromolecule was a component of sieve tube sap, rather than a contaminant leached from cell walls or cut parenchyma cells during exudate collection. The protein nature of this macromolecule was deduced from its size, lability, susceptibility to proteolytic digestion, and by the dependence of calcium-binding activity on thiol-protecting agents. This protein is distinct from the major proteins of exudate and does not appear to be related to calmodulin.
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Abbreviations
- SDS-PAGE:
-
sodium dodecyl sulphate polyacrylamide gel electrophoresis
- CBP:
-
calcium-binding protein
References
Allen, A.K. (1979) A lectin from the exudate of the fruit of the vegetable marrow (Cucurbita pepo) that has a specificity for β-1,4-linked N-acetylglucosamine oligosaccharides. Biochem. J. 183, 133–137
Allen, S.E., Grimshaw, H.M., Parkinson, J.A., Quarmby, C. (1974) Chemical Analysis of Ecological Materials. Blackwell Scientific Publications, Oxford
Anderson, J.M., Cormier, M.J. (1978) Calcium-dependent regulator of NAD kinase in higher plants. Biochem. Biophys. Res. Comm. 84, 595–602
Beyenbach, J., Weber, C., Kleinig, H. (1974) Sieve-tube proteins from Cucurbita maxima. Planta 119, 113–124
Chevallier, J., Butow, R.A. (1971) Calcium binding to the sarcoplasmic reticulum of rabbit skeletal muscle. Biochemistry 10, 2733–2737
Cronshaw, J. (1975) P-proteins. In: Phloem transport, pp. 79–115; Aronoff, S., Dainty, J., Gorham, P.R., Srivastava, L.M., Swanson, C.A., eds. Plenum Press, London
Drabikowski, W., Strzelecka-Golaszewska, H., Carafoli, E. (1974) Calcium binding proteins. Elsevier, Amsterdam
Duncan, C.J. (1976) Calcium in biological systems, S.E.B. Symposia Vol XXX. Cambridge University Press, Cambridge
Hayama, T., Shimmen, T., Tazawa, M. (1979) Participation of Ca2+ in cessation of cytoplasmic streaming induced by membrane excitation in Characeae internodal cells. Protoplasma 99, 305–321
Kleinig, H., Thönes, J., Dörr, I., Kollman, R. (1975) Filament formation in vitro of a sieve tube protein from Cucurbita maxima and Cucurbita pepo. Planta 127, 163–170
Kretsinger, R.H. (1976) Calcium-binding proteins. Annu. Rev. Biochem. 45, 239–265
Milburn, J.A. (1971) An analysis of the response in phloem exudation on application of massage to Ricinus. Planta 100, 143–154
Ross, E., Schatz, G. (1973) Assay of protein in the presence of high concentrations of sulfhydryl compounds. Anal. Biochem. 54, 304–306
Sabnis, D.D., Hart, J.W. (1978) The isolation and some properties of a lectin (Haemagglutin) from Cucurbita phloem exudate. Planta 142, 97–101
Sabnis, D.D., Hart, J.W. (1979) Heterogeneity in phloem protein complements from different species. Consequences to hypotheses concerned with P-protein function. Planta 145, 459–466
Sloan, R.T., Sabnis, D.D., Hart, J.W. (1976) The heterogeneity of phloem exudate proteins from different plants: a comparative survey of ten plants using polyacrylamide gel electrophoresis. Planta 132, 97–101
Wagner, G., Klein, K. (1978) Differential effect of calcium on chloroplast movement in Mougeotia. Photochem. Photobiol. 27, 137–140
Walker, T.S. (1972) The purification and some properties of a protein causing gelling in sieve tube exudate from Cucurbita pepo. Biochim. Biophys. Acta 257, 433–444
Wasserman, R.H., Corradino, R.A., Carafoli, E., Kretsinger, R.H., MacLennan, D.H., Siegel, F.L. (1977) Calcium binding proteins and calcium function. North Holland, Amsterdam
Weber, C., Franke, W.W., Kartenbach, J. (1974) Structure and biochemistry of phloem-proteins isolated from Cucurbita maxima. Exp. Cell. Res. 87, 79–106
Weber, K., Osborn, M. (1969) The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244, 4406–4412
Wolff, D.J., Brostrom, C.O. (1979) Properties and functions of the calcium-dependent regulator protein. In: Advances in cyclic nucleotide research, Vol. 11. pp. 27–86, Greengard, P., Robison, G.A., eds. Raven Press, New York
Ziegler, H. (1975) Nature of transported substances. In: Transport in plants I: Phloem transport, Encyclopedia of plant physiology, New Series, Vol. 1, pp. 59–100, Zimmermann, M.H., Milburn, J.A., eds. Springer Verlag, Berlin Heidelberg New York
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McEuen, A.R., Hart, J.W. & Sabnis, D.D. Calcium-binding protein in sieve tube exudate. Planta 151, 531–534 (1981). https://doi.org/10.1007/BF00387430
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DOI: https://doi.org/10.1007/BF00387430